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Clk post-transcriptional control denoises circadian transcription both temporally and spatially

Author

Listed:
  • Immanuel Lerner

    (Silberman Institute of Life Sciences, Edmund J. Safra Campus, The Hebrew University)

  • Osnat Bartok

    (Silberman Institute of Life Sciences, Edmund J. Safra Campus, The Hebrew University)

  • Victoria Wolfson

    (Silberman Institute of Life Sciences, Edmund J. Safra Campus, The Hebrew University)

  • Jerome S. Menet

    (Howard Hughes Medical Institute, Brandeis University
    Present address: Department of Biology, Texas A&M University, College Station, Texas 77843-3474, USA.)

  • Uri Weissbein

    (Silberman Institute of Life Sciences, Edmund J. Safra Campus, The Hebrew University)

  • Shaked Afik

    (Silberman Institute of Life Sciences, Edmund J. Safra Campus, The Hebrew University)

  • Daniel Haimovich

    (Silberman Institute of Life Sciences, Edmund J. Safra Campus, The Hebrew University
    School of Computer Sciences, Edmund J. Safra Campus, The Hebrew University)

  • Chen Gafni

    (Silberman Institute of Life Sciences, Edmund J. Safra Campus, The Hebrew University)

  • Nir Friedman

    (Silberman Institute of Life Sciences, Edmund J. Safra Campus, The Hebrew University
    School of Computer Sciences, Edmund J. Safra Campus, The Hebrew University)

  • Michael Rosbash

    (Howard Hughes Medical Institute, Brandeis University)

  • Sebastian Kadener

    (Silberman Institute of Life Sciences, Edmund J. Safra Campus, The Hebrew University)

Abstract

The transcription factor CLOCK (CLK) is essential for the development and maintenance of circadian rhythms in Drosophila. However, little is known about how CLK levels are controlled. Here we show that Clk mRNA is strongly regulated post-transcriptionally through its 3′ UTR. Flies expressing Clk transgenes without normal 3′ UTR exhibit variable CLK-driven transcription and circadian behaviour as well as ectopic expression of CLK-target genes in the brain. In these flies, the number of the key circadian neurons differs stochastically between individuals and within the two hemispheres of the same brain. Moreover, flies carrying Clk transgenes with deletions in the binding sites for the miRNA bantam have stochastic number of pacemaker neurons, suggesting that this miRNA mediates the deterministic expression of CLK. Overall our results demonstrate a key role of Clk post-transcriptional control in stabilizing circadian transcription, which is essential for proper development and maintenance of circadian rhythms in Drosophila.

Suggested Citation

  • Immanuel Lerner & Osnat Bartok & Victoria Wolfson & Jerome S. Menet & Uri Weissbein & Shaked Afik & Daniel Haimovich & Chen Gafni & Nir Friedman & Michael Rosbash & Sebastian Kadener, 2015. "Clk post-transcriptional control denoises circadian transcription both temporally and spatially," Nature Communications, Nature, vol. 6(1), pages 1-12, November.
    • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms8056
    DOI: 10.1038/ncomms8056
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