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RNA structure determination by solid-state NMR spectroscopy

Author

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  • Alexander Marchanka

    (Structural and Computational Biology Unit, European Molecular Biology Laboratory)

  • Bernd Simon

    (Structural and Computational Biology Unit, European Molecular Biology Laboratory)

  • Gerhard Althoff-Ospelt

    (Bruker BioSpin)

  • Teresa Carlomagno

    (Structural and Computational Biology Unit, European Molecular Biology Laboratory
    Helmholtz Zentrum für Infektionsforschung)

Abstract

Knowledge of the RNA three-dimensional structure, either in isolation or as part of RNP complexes, is fundamental to understand the mechanism of numerous cellular processes. Because of its flexibility, RNA represents a challenge for crystallization, while the large size of cellular complexes brings solution-state NMR to its limits. Here, we demonstrate an alternative approach on the basis of solid-state NMR spectroscopy. We develop a suite of experiments and RNA labeling schemes and demonstrate for the first time that ssNMR can yield a RNA structure at high-resolution. This methodology allows structural analysis of segmentally labelled RNA stretches in high-molecular weight cellular machines—independent of their ability to crystallize— and opens the way to mechanistic studies of currently difficult-to-access RNA-protein assemblies.

Suggested Citation

  • Alexander Marchanka & Bernd Simon & Gerhard Althoff-Ospelt & Teresa Carlomagno, 2015. "RNA structure determination by solid-state NMR spectroscopy," Nature Communications, Nature, vol. 6(1), pages 1-7, November.
    • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms8024
    DOI: 10.1038/ncomms8024
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