IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v6y2015i1d10.1038_ncomms8005.html
   My bibliography  Save this article

Topological control of the Caulobacter cell cycle circuitry by a polarized single-domain PAS protein

Author

Listed:
  • Stefano Sanselicio

    (Institute of Genetics and Genomics in Geneva (iGE3), Faculty of Medicine/CMU, University of Geneva)

  • Matthieu Bergé

    (Institute of Genetics and Genomics in Geneva (iGE3), Faculty of Medicine/CMU, University of Geneva)

  • Laurence Théraulaz

    (Institute of Genetics and Genomics in Geneva (iGE3), Faculty of Medicine/CMU, University of Geneva)

  • Sunish Kumar Radhakrishnan

    (Institute of Genetics and Genomics in Geneva (iGE3), Faculty of Medicine/CMU, University of Geneva
    Present address: School of Biology, Indian Institute of Science Education and Research, Thiruvananthapuram 695016, India)

  • Patrick H. Viollier

    (Institute of Genetics and Genomics in Geneva (iGE3), Faculty of Medicine/CMU, University of Geneva)

Abstract

Despite the myriad of different sensory domains encoded in bacteria, only a few types are known to control the cell cycle. Here we use a forward genetic screen for Caulobacter crescentus motility mutants to identify a conserved single-domain PAS (Per-Arnt-Sim) protein (MopJ) with pleiotropic regulatory functions. MopJ promotes re-accumulation of the master cell cycle regulator CtrA after its proteolytic destruction is triggered by the DivJ kinase at the G1-S transition. MopJ and CtrA syntheses are coordinately induced in S-phase, followed by the sequestration of MopJ to cell poles in Caulobacter. Polarization requires Caulobacter DivJ and the PopZ polar organizer. MopJ interacts with DivJ and influences the localization and activity of downstream cell cycle effectors. Because MopJ abundance is upregulated in stationary phase and by the alarmone (p)ppGpp, conserved systemic signals acting on the cell cycle and growth phase control are genetically integrated through this conserved single PAS-domain protein.

Suggested Citation

  • Stefano Sanselicio & Matthieu Bergé & Laurence Théraulaz & Sunish Kumar Radhakrishnan & Patrick H. Viollier, 2015. "Topological control of the Caulobacter cell cycle circuitry by a polarized single-domain PAS protein," Nature Communications, Nature, vol. 6(1), pages 1-14, November.
    • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms8005
    DOI: 10.1038/ncomms8005
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/ncomms8005
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/ncomms8005?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms8005. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.