Author
Listed:
- Bogumil J. Karas
(Synthetic Biology and Bioenergy Group, J. Craig Venter Institute
Present address: Designer Microbes Inc., London, Ontario, Canada.)
- Rachel E. Diner
(Microbial and Environmental Genomics Group, J. Craig Venter Institute
Scripps Institution of Oceanography, University of California San Diego)
- Stephane C. Lefebvre
(Microbial and Environmental Genomics Group, J. Craig Venter Institute)
- Jeff McQuaid
(Microbial and Environmental Genomics Group, J. Craig Venter Institute)
- Alex P.R. Phillips
(Synthetic Biology and Bioenergy Group, J. Craig Venter Institute)
- Chari M. Noddings
(Synthetic Biology and Bioenergy Group, J. Craig Venter Institute)
- John K. Brunson
(Synthetic Biology and Bioenergy Group, J. Craig Venter Institute)
- Ruben E. Valas
(Microbial and Environmental Genomics Group, J. Craig Venter Institute)
- Thomas J. Deerinck
(National Center for Microscopy and Imaging Research, University of California, San Diego)
- Jelena Jablanovic
(Microbial and Environmental Genomics Group, J. Craig Venter Institute)
- Jeroen T.F. Gillard
(Microbial and Environmental Genomics Group, J. Craig Venter Institute)
- Karen Beeri
(Microbial and Environmental Genomics Group, J. Craig Venter Institute)
- Mark H. Ellisman
(National Center for Microscopy and Imaging Research, University of California, San Diego)
- John I. Glass
(Synthetic Biology and Bioenergy Group, J. Craig Venter Institute)
- Clyde A. Hutchison III
(Synthetic Biology and Bioenergy Group, J. Craig Venter Institute)
- Hamilton O. Smith
(Synthetic Biology and Bioenergy Group, J. Craig Venter Institute)
- J. Craig Venter
(Synthetic Biology and Bioenergy Group, J. Craig Venter Institute
Microbial and Environmental Genomics Group, J. Craig Venter Institute)
- Andrew E. Allen
(Microbial and Environmental Genomics Group, J. Craig Venter Institute
Scripps Institution of Oceanography, University of California San Diego)
- Christopher L. Dupont
(Microbial and Environmental Genomics Group, J. Craig Venter Institute)
- Philip D. Weyman
(Synthetic Biology and Bioenergy Group, J. Craig Venter Institute)
Abstract
Eukaryotic microalgae hold great promise for the bioproduction of fuels and higher value chemicals. However, compared with model genetic organisms such as Escherichia coli and Saccharomyces cerevisiae, characterization of the complex biology and biochemistry of algae and strain improvement has been hampered by the inefficient genetic tools. To date, many algal species are transformable only via particle bombardment, and the introduced DNA is integrated randomly into the nuclear genome. Here we describe the first nuclear episomal vector for diatoms and a plasmid delivery method via conjugation from Escherichia coli to the diatoms Phaeodactylum tricornutum and Thalassiosira pseudonana. We identify a yeast-derived sequence that enables stable episome replication in these diatoms even in the absence of antibiotic selection and show that episomes are maintained as closed circles at copy number equivalent to native chromosomes. This highly efficient genetic system facilitates high-throughput functional characterization of algal genes and accelerates molecular phytoplankton research.
Suggested Citation
Bogumil J. Karas & Rachel E. Diner & Stephane C. Lefebvre & Jeff McQuaid & Alex P.R. Phillips & Chari M. Noddings & John K. Brunson & Ruben E. Valas & Thomas J. Deerinck & Jelena Jablanovic & Jeroen T, 2015.
"Designer diatom episomes delivered by bacterial conjugation,"
Nature Communications, Nature, vol. 6(1), pages 1-10, November.
Handle:
RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms7925
DOI: 10.1038/ncomms7925
Download full text from publisher
Corrections
All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms7925. See general information about how to correct material in RePEc.
If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.
We have no bibliographic references for this item. You can help adding them by using this form .
If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.
For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .
Please note that corrections may take a couple of weeks to filter through
the various RePEc services.