Author
Listed:
- Kyungjin Boo
(Creative Research Initiative Center for Chromatin Dynamics, School of Biological Sciences, Seoul National University)
- Jinhyuk Bhin
(POSTECH)
- Yoon Jeon
(Research Institute, Graduate School of Cancer Science and Policy, National Cancer Center)
- Joomyung Kim
(Creative Research Initiative Center for Chromatin Dynamics, School of Biological Sciences, Seoul National University)
- Hi-Jai R. Shin
(Creative Research Initiative Center for Chromatin Dynamics, School of Biological Sciences, Seoul National University)
- Jong-Eun Park
(Institute for Basic Science, School of Biological Sciences, Seoul National University)
- Kyeongkyu Kim
(Creative Research Initiative Center for Chromatin Dynamics, School of Biological Sciences, Seoul National University)
- Chang Rok Kim
(Creative Research Initiative Center for Chromatin Dynamics, School of Biological Sciences, Seoul National University)
- Hyonchol Jang
(Research Institute, Graduate School of Cancer Science and Policy, National Cancer Center)
- In-Hoo Kim
(Research Institute, Graduate School of Cancer Science and Policy, National Cancer Center)
- V. Narry Kim
(Institute for Basic Science, School of Biological Sciences, Seoul National University)
- Daehee Hwang
(POSTECH
Institute for Basic Science, DGIST)
- Ho Lee
(Research Institute, Graduate School of Cancer Science and Policy, National Cancer Center)
- Sung Hee Baek
(Creative Research Initiative Center for Chromatin Dynamics, School of Biological Sciences, Seoul National University)
Abstract
The actions of transcription factors, chromatin modifiers and noncoding RNAs are crucial for the programming of cell states. Although the importance of various epigenetic machineries for controlling pluripotency of embryonic stem (ES) cells has been previously studied, how chromatin modifiers cooperate with specific transcription factors still remains largely elusive. Here, we find that Pontin chromatin remodelling factor plays an essential role as a coactivator for Oct4 for maintenance of pluripotency in mouse ES cells. Genome-wide analyses reveal that Pontin and Oct4 share a substantial set of target genes involved in ES cell maintenance. Intriguingly, we find that the Oct4-dependent coactivator function of Pontin extends to the transcription of large intergenic noncoding RNAs (lincRNAs) and in particular linc1253, a lineage programme repressing lincRNA, is a Pontin-dependent Oct4 target lincRNA. Together, our findings demonstrate that the Oct4-Pontin module plays critical roles in the regulation of genes involved in ES cell fate determination.
Suggested Citation
Kyungjin Boo & Jinhyuk Bhin & Yoon Jeon & Joomyung Kim & Hi-Jai R. Shin & Jong-Eun Park & Kyeongkyu Kim & Chang Rok Kim & Hyonchol Jang & In-Hoo Kim & V. Narry Kim & Daehee Hwang & Ho Lee & Sung Hee B, 2015.
"Pontin functions as an essential coactivator for Oct4-dependent lincRNA expression in mouse embryonic stem cells,"
Nature Communications, Nature, vol. 6(1), pages 1-16, November.
Handle:
RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms7810
DOI: 10.1038/ncomms7810
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