IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v6y2015i1d10.1038_ncomms7751.html
   My bibliography  Save this article

The clathrin adaptor AP-1 complex and Arf1 regulate planar cell polarity in vivo

Author

Listed:
  • Jose Maria Carvajal-Gonzalez

    (Icahn School of Medicine at Mount Sinai)

  • Sophie Balmer

    (Icahn School of Medicine at Mount Sinai)

  • Meg Mendoza

    (Program in Developmental and Stem Cell Biology, The Hospital for Sick Children, University of Toronto)

  • Aurore Dussert

    (Icahn School of Medicine at Mount Sinai)

  • Giovanna Collu

    (Icahn School of Medicine at Mount Sinai)

  • Angel-Carlos Roman

    (Instituto Cajal, CSIC)

  • Ursula Weber

    (Icahn School of Medicine at Mount Sinai)

  • Brian Ciruna

    (Program in Developmental and Stem Cell Biology, The Hospital for Sick Children, University of Toronto)

  • Marek Mlodzik

    (Icahn School of Medicine at Mount Sinai)

Abstract

A key step in generating planar cell polarity (PCP) is the formation of restricted junctional domains containing Frizzled/Dishevelled/Diego (Fz/Dsh/Dgo) or Van Gogh/Prickle (Vang/Pk) complexes within the same cell, stabilized via Flamingo (Fmi) across cell membranes. Although models have been proposed for how these complexes acquire and maintain their polarized localization, the machinery involved in moving core PCP proteins around cells remains unknown. We describe the AP-1 adaptor complex and Arf1 as major regulators of PCP protein trafficking in vivo. AP-1 and Arf1 disruption affects the accumulation of Fz/Fmi and Vang/Fmi complexes in the proximo–distal axis, producing severe PCP phenotypes. Using novel tools, we demonstrate a direct and specific Arf1 involvement in Fz trafficking in vivo. Moreover, we uncover a conserved Arf1 PCP function in vertebrates. Our data support a model whereby the trafficking machinery plays an important part during PCP establishment, promoting formation of polarized PCP-core complexes in vivo.

Suggested Citation

  • Jose Maria Carvajal-Gonzalez & Sophie Balmer & Meg Mendoza & Aurore Dussert & Giovanna Collu & Angel-Carlos Roman & Ursula Weber & Brian Ciruna & Marek Mlodzik, 2015. "The clathrin adaptor AP-1 complex and Arf1 regulate planar cell polarity in vivo," Nature Communications, Nature, vol. 6(1), pages 1-15, November.
    • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms7751
    DOI: 10.1038/ncomms7751
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/ncomms7751
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/ncomms7751?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Giulia Fasano & Valentina Muto & Francesca Clementina Radio & Martina Venditti & Niloufar Mosaddeghzadeh & Simona Coppola & Graziamaria Paradisi & Erika Zara & Farhad Bazgir & Alban Ziegler & Giovanni, 2022. "Dominant ARF3 variants disrupt Golgi integrity and cause a neurodevelopmental disorder recapitulated in zebrafish," Nature Communications, Nature, vol. 13(1), pages 1-29, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms7751. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.