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A balance between membrane elasticity and polymerization energy sets the shape of spherical clathrin coats

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  • Mohammed Saleem

    (University of Geneva
    Present address: Department of Life Science, National Institute of Technology, Rourkela, Odisha, India)

  • Sandrine Morlot

    (University of Geneva
    Present address: Department of Development and Stem Cells Biology, IGBMC, Illkirch, F-67404, France)

  • Annika Hohendahl

    (University of Geneva)

  • John Manzi

    (PCC, UMR168, Institut Curie/CNRS/Université Pierre et Marie Curie)

  • Martin Lenz

    (University LPTMS, UMR 8626, CNRS/Université Paris Sud)

  • Aurélien Roux

    (University of Geneva
    Swiss National Centre for Competence in Research Programme Chemical Biology
    Present address: Department of Development and Stem Cells Biology, IGBMC, Illkirch, F-67404, France)

Abstract

In endocytosis, scaffolding is one of the mechanisms to create membrane curvature by moulding the membrane into the spherical shape of the clathrin cage. However, the impact of membrane elastic parameters on the assembly and shape of clathrin lattices has never been experimentally evaluated. Here, we show that membrane tension opposes clathrin polymerization. We reconstitute clathrin budding in vitro with giant unilamellar vesicles (GUVs), purified adaptors and clathrin. By changing the osmotic conditions, we find that clathrin coats cause extensive budding of GUVs under low membrane tension while polymerizing into shallow pits under moderate tension. High tension fully inhibits polymerization. Theoretically, we predict the tension values for which transitions between different clathrin coat shapes occur. We measure the changes in membrane tension during clathrin polymerization, and use our theoretical framework to estimate the polymerization energy from these data. Our results show that membrane tension controls clathrin-mediated budding by varying the membrane budding energy.

Suggested Citation

  • Mohammed Saleem & Sandrine Morlot & Annika Hohendahl & John Manzi & Martin Lenz & Aurélien Roux, 2015. "A balance between membrane elasticity and polymerization energy sets the shape of spherical clathrin coats," Nature Communications, Nature, vol. 6(1), pages 1-10, May.
    • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms7249
    DOI: 10.1038/ncomms7249
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    Cited by:

    1. Meiyan Jin & Cyna Shirazinejad & Bowen Wang & Amy Yan & Johannes Schöneberg & Srigokul Upadhyayula & Ke Xu & David G. Drubin, 2022. "Branched actin networks are organized for asymmetric force production during clathrin-mediated endocytosis in mammalian cells," Nature Communications, Nature, vol. 13(1), pages 1-12, December.

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