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PFKFB4 controls embryonic patterning via Akt signalling independently of glycolysis

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  • Caterina Pegoraro

    (Université Paris Sud
    Institut Curie, Centre Universitaire
    CNRS UMR3347, Centre Universitaire
    INSERM U1021, Centre Universitaire)

  • Ana Leonor Figueiredo

    (Université Paris Sud
    Institut Curie, Centre Universitaire
    CNRS UMR3347, Centre Universitaire
    INSERM U1021, Centre Universitaire)

  • Frédérique Maczkowiak

    (Université Paris Sud
    Institut Curie, Centre Universitaire
    CNRS UMR3347, Centre Universitaire
    INSERM U1021, Centre Universitaire)

  • Celio Pouponnot

    (Université Paris Sud
    Institut Curie, Centre Universitaire
    CNRS UMR3347, Centre Universitaire
    INSERM U1021, Centre Universitaire)

  • Alain Eychène

    (Université Paris Sud
    Institut Curie, Centre Universitaire
    CNRS UMR3347, Centre Universitaire
    INSERM U1021, Centre Universitaire)

  • Anne H. Monsoro-Burq

    (Université Paris Sud
    Institut Curie, Centre Universitaire
    CNRS UMR3347, Centre Universitaire
    INSERM U1021, Centre Universitaire)

Abstract

How metabolism regulators play roles during early development remains elusive. Here we show that PFKFB4 (6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4), a glycolysis regulator, is critical for controlling dorsal ectoderm global patterning in gastrulating frog embryos via a non-glycolytic function. PFKFB4 is required for dorsal ectoderm progenitors to proceed towards more specified fates including neural and non-neural ectoderm, neural crest or placodes. This function is mediated by Akt signalling, a major pathway that integrates cell homeostasis and survival parameters. Restoring Akt signalling rescues the loss of PFKFB4 in vivo. In contrast, glycolysis is not essential for frog development at this stage. Our study reveals the existence of a PFKFB4–Akt checkpoint that links cell homeostasis to the ability of progenitor cells to undergo differentiation, and uncovers glycolysis-independent functions of PFKFB4.

Suggested Citation

  • Caterina Pegoraro & Ana Leonor Figueiredo & Frédérique Maczkowiak & Celio Pouponnot & Alain Eychène & Anne H. Monsoro-Burq, 2015. "PFKFB4 controls embryonic patterning via Akt signalling independently of glycolysis," Nature Communications, Nature, vol. 6(1), pages 1-9, May.
    • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms6953
    DOI: 10.1038/ncomms6953
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    Cited by:

    1. Jean-Louis Plouhinec & Sofía Medina-Ruiz & Caroline Borday & Elsa Bernard & Jean-Philippe Vert & Michael B Eisen & Richard M Harland & Anne H Monsoro-Burq, 2017. "A molecular atlas of the developing ectoderm defines neural, neural crest, placode, and nonneural progenitor identity in vertebrates," PLOS Biology, Public Library of Science, vol. 15(10), pages 1-44, October.

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