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AtPHT4;4 is a chloroplast-localized ascorbate transporter in Arabidopsis

Author

Listed:
  • Takaaki Miyaji

    (Advanced Science Research Center, Okayama University)

  • Takashi Kuromori

    (Gene Discovery Research Group, RIKEN Center for Sustainable Resource Science)

  • Yu Takeuchi

    (Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences)

  • Naoki Yamaji

    (Institute of Plant Science and Resources, Okayama University)

  • Kengo Yokosho

    (Institute of Plant Science and Resources, Okayama University)

  • Atsushi Shimazawa

    (Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences)

  • Eriko Sugimoto

    (Gene Discovery Research Group, RIKEN Center for Sustainable Resource Science)

  • Hiroshi Omote

    (Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences)

  • Jian Feng Ma

    (Institute of Plant Science and Resources, Okayama University)

  • Kazuo Shinozaki

    (Gene Discovery Research Group, RIKEN Center for Sustainable Resource Science)

  • Yoshinori Moriyama

    (Advanced Science Research Center, Okayama University
    Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences)

Abstract

Ascorbate is an antioxidant and coenzyme for various metabolic reactions in vivo. In plant chloroplasts, high ascorbate levels are required to overcome photoinhibition caused by strong light. However, ascorbate is synthesized in the mitochondria and the molecular mechanisms underlying ascorbate transport into chloroplasts are unknown. Here we show that AtPHT4;4, a member of the phosphate transporter 4 family of Arabidopsis thaliana, functions as an ascorbate transporter. In vitro analysis shows that proteoliposomes containing the purified AtPHT4;4 protein exhibit membrane potential- and Cl−-dependent ascorbate uptake. The AtPHT4;4 protein is abundantly expressed in the chloroplast envelope membrane. Knockout of AtPHT4;4 results in decreased levels of the reduced form of ascorbate in the leaves and the heat dissipation process of excessive energy during photosynthesis is compromised. Taken together, these observations indicate that the AtPHT4;4 protein is an ascorbate transporter at the chloroplast envelope membrane, which may be required for tolerance to strong light stress.

Suggested Citation

  • Takaaki Miyaji & Takashi Kuromori & Yu Takeuchi & Naoki Yamaji & Kengo Yokosho & Atsushi Shimazawa & Eriko Sugimoto & Hiroshi Omote & Jian Feng Ma & Kazuo Shinozaki & Yoshinori Moriyama, 2015. "AtPHT4;4 is a chloroplast-localized ascorbate transporter in Arabidopsis," Nature Communications, Nature, vol. 6(1), pages 1-11, May.
    • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms6928
    DOI: 10.1038/ncomms6928
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    Cited by:

    1. Namiki Mitani-Ueno & Naoki Yamaji & Sheng Huang & Yuma Yoshioka & Takaaki Miyaji & Jian Feng Ma, 2023. "A silicon transporter gene required for healthy growth of rice on land," Nature Communications, Nature, vol. 14(1), pages 1-10, December.
    2. Hye-In Nam & Zaigham Shahzad & Yanniv Dorone & Sophie Clowez & Kangmei Zhao & Nadia Bouain & Katerina S. Lay-Pruitt & Huikyong Cho & Seung Y. Rhee & Hatem Rouached, 2021. "Interdependent iron and phosphorus availability controls photosynthesis through retrograde signaling," Nature Communications, Nature, vol. 12(1), pages 1-13, December.

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