IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v6y2015i1d10.1038_ncomms10095.html
   My bibliography  Save this article

Structured line illumination Raman microscopy

Author

Listed:
  • Kozue Watanabe

    (Graduate School of Engineering, Osaka University)

  • Almar F. Palonpon

    (Graduate School of Engineering, Osaka University)

  • Nicholas I. Smith

    (Immunology Frontier Research Center, Osaka University)

  • Liang-da Chiu

    (Graduate School of Engineering, Osaka University)

  • Atsushi Kasai

    (Laboratory of Molecular Neuropharmacology, Graduate School of Pharmaceutical Sciences, Osaka University
    Interdisciplinary Program for Biomedical Sciences, Institute for Academic Initiatives, Osaka University)

  • Hitoshi Hashimoto

    (Laboratory of Molecular Neuropharmacology, Graduate School of Pharmaceutical Sciences, Osaka University
    iPS Cell-based Research Project on Brain Neuropharmacology and Toxicology, Graduate school of Pharmaceutical Sciences, Osaka University
    Molecular Research Center for Children’s Mental Development, United Graduate School of Child Development, Osaka University, Kanazawa University, Hamamatsu University School of Medicine, Chiba University and University of Fukui)

  • Satoshi Kawata

    (Graduate School of Engineering, Osaka University)

  • Katsumasa Fujita

    (Graduate School of Engineering, Osaka University)

Abstract

In the last couple of decades, the spatial resolution in optical microscopy has increased to unprecedented levels by exploiting the fluorescence properties of the probe. At about the same time, Raman imaging techniques have emerged as a way to image inherent chemical information in a sample without using fluorescent probes. However, in many applications, the achievable resolution is limited to about half the wavelength of excitation light. Here we report the use of structured illumination to increase the spatial resolution of label-free spontaneous Raman microscopy, generating highly detailed spatial contrast from the ensemble of molecular information in the sample. Using structured line illumination in slit-scanning Raman microscopy, we demonstrate a marked improvement in spatial resolution and show the applicability to a range of samples, including both biological and inorganic chemical component mapping. This technique is expected to contribute towards greater understanding of chemical component distributions in organic and inorganic materials.

Suggested Citation

  • Kozue Watanabe & Almar F. Palonpon & Nicholas I. Smith & Liang-da Chiu & Atsushi Kasai & Hitoshi Hashimoto & Satoshi Kawata & Katsumasa Fujita, 2015. "Structured line illumination Raman microscopy," Nature Communications, Nature, vol. 6(1), pages 1-8, December.
    • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms10095
    DOI: 10.1038/ncomms10095
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/ncomms10095
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/ncomms10095?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms10095. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.