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Dynamic labelling of neural connections in multiple colours by trans-synaptic fluorescence complementation

Author

Listed:
  • Lindsey J. Macpherson

    (Columbia University)

  • Emanuela E. Zaharieva

    (Northwestern University)

  • Patrick J. Kearney

    (Northwestern University
    Current address: University of Massachusetts Medical School, 55N Lake Avenue, Worcester, Massachusetts 01655, USA)

  • Michael H. Alpert

    (Northwestern University)

  • Tzu-Yang Lin

    (Section on Neuronal Connectivity, Laboratory of Gene Regulation and Development, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health
    Current address: Graduate Institute of Life Sciences, National Defense Medical Center, Taipei 114, Taiwan)

  • Zeynep Turan

    (Columbia University)

  • Chi-Hon Lee

    (Section on Neuronal Connectivity, Laboratory of Gene Regulation and Development, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health)

  • Marco Gallio

    (Northwestern University)

Abstract

Determining the pattern of activity of individual connections within a neural circuit could provide insights into the computational processes that underlie brain function. Here, we develop new strategies to label active synapses by trans-synaptic fluorescence complementation in Drosophila. First, we demonstrate that a synaptobrevin-GRASP chimera functions as a powerful activity-dependent marker for synapses in vivo. Next, we create cyan and yellow variants, achieving activity-dependent, multi-colour fluorescence reconstitution across synapses (X-RASP). Our system allows for the first time retrospective labelling of synapses (rather than whole neurons) based on their activity, in multiple colours, in the same animal. As individual synapses often act as computational units in the brain, our method will promote the design of experiments that are not possible using existing techniques. Moreover, our strategies are easily adaptable to circuit mapping in any genetic system.

Suggested Citation

  • Lindsey J. Macpherson & Emanuela E. Zaharieva & Patrick J. Kearney & Michael H. Alpert & Tzu-Yang Lin & Zeynep Turan & Chi-Hon Lee & Marco Gallio, 2015. "Dynamic labelling of neural connections in multiple colours by trans-synaptic fluorescence complementation," Nature Communications, Nature, vol. 6(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms10024
    DOI: 10.1038/ncomms10024
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    Cited by:

    1. Akshay Patel & Yicong Wu & Xiaofei Han & Yijun Su & Tim Maugel & Hari Shroff & Sougata Roy, 2022. "Cytonemes coordinate asymmetric signaling and organization in the Drosophila muscle progenitor niche," Nature Communications, Nature, vol. 13(1), pages 1-17, December.
    2. Mami Nakamizo-Dojo & Kenichi Ishii & Jiro Yoshino & Masato Tsuji & Kazuo Emoto, 2023. "Descending GABAergic pathway links brain sugar-sensing to peripheral nociceptive gating in Drosophila," Nature Communications, Nature, vol. 14(1), pages 1-18, December.

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