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The CDC13-STN1-TEN1 complex stimulates Pol α activity by promoting RNA priming and primase-to-polymerase switch

Author

Listed:
  • Neal F. Lue

    (W. R. Hearst Microbiology Research Center, Weill Medical College of Cornell University)

  • Jamie Chan

    (W. R. Hearst Microbiology Research Center, Weill Medical College of Cornell University)

  • Woodring E. Wright

    (University of Texas Southwestern Medical Center)

  • Jerard Hurwitz

    (Molecular Biology Program, Memorial Sloan-Kettering Cancer Center)

Abstract

Emerging evidence suggests that Cdc13-Stn1-Ten1 (CST), an RPA-like ssDNA-binding complex, may regulate primase-Pol α (PP) activity at telomeres constitutively, and at other genomic locations under conditions of replication stress. Here we examine the mechanisms of PP stimulation by CST using purified complexes derived from Candida glabrata. While CST does not enhance isolated DNA polymerase activity, it substantially augments both primase activity and primase-to-polymerase switching. CST also simultaneously shortens the RNA and lengthens the DNA in the chimeric products. Stn1, the most conserved subunit of CST, is alone capable of PP stimulation. Both the N-terminal OB fold and the C-terminal winged-helix domains of Stn1 can bind to the Pol12 subunit of the PP complex and stimulate PP activity. Our findings provide mechanistic insights on a well-conserved pathway of PP regulation that is critical for genome stability.

Suggested Citation

  • Neal F. Lue & Jamie Chan & Woodring E. Wright & Jerard Hurwitz, 2014. "The CDC13-STN1-TEN1 complex stimulates Pol α activity by promoting RNA priming and primase-to-polymerase switch," Nature Communications, Nature, vol. 5(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms6762
    DOI: 10.1038/ncomms6762
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    Cited by:

    1. Kai-Hang Lei & Han-Lin Yang & Hao-Yen Chang & Hsin-Yi Yeh & Dinh Duc Nguyen & Tzu-Yu Lee & Xinxing Lyu & Megan Chastain & Weihang Chai & Hung-Wen Li & Peter Chi, 2021. "Crosstalk between CST and RPA regulates RAD51 activity during replication stress," Nature Communications, Nature, vol. 12(1), pages 1-15, December.

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