IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v5y2014i1d10.1038_ncomms6728.html
   My bibliography  Save this article

Transformation of intestinal stem cells into gastric stem cells on loss of transcription factor Cdx2

Author

Listed:
  • Salvatore Simmini

    (Hubrecht Institute and UMC Utrecht)

  • Monika Bialecka

    (Hubrecht Institute and UMC Utrecht
    Present address: University of Cambridge, Department of Physiology, Development & Neuroscience and Gurdon Institute Downing Street, Cambridge CB2 3DY, UK)

  • Meritxell Huch

    (Hubrecht Institute and UMC Utrecht
    Present address: Wellcome Trust/Cancer Research UK Gurdon Institute, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK)

  • Lennart Kester

    (Hubrecht Institute and UMC Utrecht)

  • Marc van de Wetering

    (Hubrecht Institute and UMC Utrecht)

  • Toshiro Sato

    (Hubrecht Institute and UMC Utrecht
    Present address: Department of Gastroenterology, School of Medicine, Keio University Tokyo, Japan)

  • Felix Beck

    (University of Leicester)

  • Alexander van Oudenaarden

    (Hubrecht Institute and UMC Utrecht)

  • Hans Clevers

    (Hubrecht Institute and UMC Utrecht)

  • Jacqueline Deschamps

    (Hubrecht Institute and UMC Utrecht)

Abstract

The endodermal lining of the adult gastro-intestinal tract harbours stem cells that are responsible for the day-to-day regeneration of the epithelium. Stem cells residing in the pyloric glands of the stomach and in the small intestinal crypts differ in their differentiation programme and in the gene repertoire that they express. Both types of stem cells have been shown to grow from single cells into 3D structures (organoids) in vitro. We show that single adult Lgr5-positive stem cells, isolated from small intestinal organoids, require Cdx2 to maintain their intestinal identity and are converted cell-autonomously into pyloric stem cells in the absence of this transcription factor. Clonal descendants of Cdx2null small intestinal stem cells enter the gastric differentiation program instead of producing intestinal derivatives. We show that the intestinal genetic programme is critically dependent on the single transcription factor encoding gene Cdx2.

Suggested Citation

  • Salvatore Simmini & Monika Bialecka & Meritxell Huch & Lennart Kester & Marc van de Wetering & Toshiro Sato & Felix Beck & Alexander van Oudenaarden & Hans Clevers & Jacqueline Deschamps, 2014. "Transformation of intestinal stem cells into gastric stem cells on loss of transcription factor Cdx2," Nature Communications, Nature, vol. 5(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms6728
    DOI: 10.1038/ncomms6728
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/ncomms6728
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/ncomms6728?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Kathleen Shah & Muralidhara Rao Maradana & M. Joaquina Delàs & Amina Metidji & Frederike Graelmann & Miriam Llorian & Probir Chakravarty & Ying Li & Mauro Tolaini & Michael Shapiro & Gavin Kelly & Chr, 2022. "Cell-intrinsic Aryl Hydrocarbon Receptor signalling is required for the resolution of injury-induced colonic stem cells," Nature Communications, Nature, vol. 13(1), pages 1-16, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms6728. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.