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BMP signalling regulates the pre-implantation development of extra-embryonic cell lineages in the mouse embryo

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  • Sarah J. L. Graham

    (Development and Neuroscience, University of Cambridge)

  • Krzysztof B. Wicher

    (Development and Neuroscience, University of Cambridge
    Present address: Department of Antibody Discovery and Protein Engineering, MedImmune, Milstein Building, Granta Park, Cambridge CB21 6GH, UK)

  • Agnieszka Jedrusik

    (Development and Neuroscience, University of Cambridge)

  • Guoji Guo

    (Developmental Cellomics Laboratory, Genome Institute of Singapore
    National University of Singapore)

  • Wishva Herath

    (Developmental Cellomics Laboratory, Genome Institute of Singapore)

  • Paul Robson

    (Developmental Cellomics Laboratory, Genome Institute of Singapore
    National University of Singapore)

  • Magdalena Zernicka-Goetz

    (Development and Neuroscience, University of Cambridge)

Abstract

Pre-implantation development requires the specification and organization of embryonic and extra-embryonic lineages. The separation of these lineages takes place when asymmetric divisions generate inside and outside cells that differ in polarity, position and fate. Here we assess the global transcriptional identities of these precursor cells to gain insight into the molecular mechanisms regulating lineage segregation. Unexpectedly, this reveals that complementary components of the bone morphogenetic protein (BMP) signalling pathway are already differentially expressed after the first wave of asymmetric divisions. We investigate the role of BMP signalling by expressing dominant negative forms of Smad4 and Bmpr2, by downregulating the pathway using RNA interference against BMP ligands and by applying three different BMP inhibitors at distinct stages. This reveals that BMP signalling regulates the correct development of both extra-embryonic lineages, primitive endoderm and trophectoderm, but not the embryonic lineage, before implantation. Together, these findings indicate multiple roles of BMP signalling in the early mouse embryo.

Suggested Citation

  • Sarah J. L. Graham & Krzysztof B. Wicher & Agnieszka Jedrusik & Guoji Guo & Wishva Herath & Paul Robson & Magdalena Zernicka-Goetz, 2014. "BMP signalling regulates the pre-implantation development of extra-embryonic cell lineages in the mouse embryo," Nature Communications, Nature, vol. 5(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms6667
    DOI: 10.1038/ncomms6667
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    Cited by:

    1. Sabine C Fischer & Elena Corujo-Simon & Joaquin Lilao-Garzon & Ernst H K Stelzer & Silvia Muñoz-Descalzo, 2020. "The transition from local to global patterns governs the differentiation of mouse blastocysts," PLOS ONE, Public Library of Science, vol. 15(5), pages 1-29, May.

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