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Dynamic SUMO modification regulates mitotic chromosome assembly and cell cycle progression in Caenorhabditis elegans

Author

Listed:
  • Federico Pelisch

    (Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee)

  • Remi Sonneville

    (Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee
    Present address: MRC Protein Phosphorylation and ubiquitylation Unit, University of Dundee, Dundee DD1 5EH, UK;)

  • Ehsan Pourkarimi

    (Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee
    Present address: Memorial Sloan Kettering Cancer Center, New York NY 10065, USA;)

  • Ana Agostinho

    (Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee
    Present address: Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm S-171 77, Sweden)

  • J. Julian Blow

    (Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee)

  • Anton Gartner

    (Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee)

  • Ronald T. Hay

    (Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee)

Abstract

The small ubiquitin-like modifier (SUMO), initially characterized as a suppressor of a mutation in the gene encoding the centromeric protein MIF2, is involved in many aspects of cell cycle regulation. The dynamics of conjugation and deconjugation and the role of SUMO during the cell cycle remain unexplored. Here we used Caenorhabditis elegans to establish the contribution of SUMO to a timely and accurate cell division. Chromatin-associated SUMO conjugates increase during metaphase but decrease rapidly during anaphase. Accumulation of SUMO conjugates on the metaphase plate and proper chromosome alignment depend on the SUMO E2 conjugating enzyme UBC-9 and SUMO E3 ligase PIASGEI-17. Deconjugation is achieved by the SUMO protease ULP-4 and is crucial for correct progression through the cell cycle. Moreover, ULP-4 is necessary for Aurora BAIR-2 extraction from chromatin and relocation to the spindle mid-zone. Our results show that dynamic SUMO conjugation plays a role in cell cycle progression.

Suggested Citation

  • Federico Pelisch & Remi Sonneville & Ehsan Pourkarimi & Ana Agostinho & J. Julian Blow & Anton Gartner & Ronald T. Hay, 2014. "Dynamic SUMO modification regulates mitotic chromosome assembly and cell cycle progression in Caenorhabditis elegans," Nature Communications, Nature, vol. 5(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms6485
    DOI: 10.1038/ncomms6485
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    Cited by:

    1. Joana S. Rodrigues & Miguel Chenlo & Susana B. Bravo & Sihara Perez-Romero & Maria Suarez-Fariña & Tomas Sobrino & Rebeca Sanz-Pamplona & Román González-Prieto & Manuel Narciso Blanco Freire & Ruben N, 2024. "dsRNAi-mediated silencing of PIAS2beta specifically kills anaplastic carcinomas by mitotic catastrophe," Nature Communications, Nature, vol. 15(1), pages 1-30, December.

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