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Implementation of the CRISPR-Cas9 system in fission yeast

Author

Listed:
  • Jake Z. Jacobs

    (Rutgers, the State University of New Jersey)

  • Keith M. Ciccaglione

    (Rutgers, the State University of New Jersey)

  • Vincent Tournier

    (Rutgers, the State University of New Jersey)

  • Mikel Zaratiegui

    (Rutgers, the State University of New Jersey)

Abstract

Application of the CRISPR-Cas9 genome editing system in the model organism Schizosaccharomyces pombe has been hampered by the lack of constructs to express RNA of arbitrary sequence. Here we present expression constructs that use the promoter/leader RNA of K RNA (rrk1) and a ribozyme to produce the targeting guide RNA. Together with constitutive expression of Cas9, this system achieves selection-free specific mutagenesis with efficiencies approaching 100%. The rrk1 CRISPR-Cas9 method enables rapid and efficient genome manipulation and unlocks the CRISPR toolset for use in fission yeast.

Suggested Citation

  • Jake Z. Jacobs & Keith M. Ciccaglione & Vincent Tournier & Mikel Zaratiegui, 2014. "Implementation of the CRISPR-Cas9 system in fission yeast," Nature Communications, Nature, vol. 5(1), pages 1-5, December.
    • Handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms6344
    DOI: 10.1038/ncomms6344
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    Cited by:

    1. Shanmugam, Sabarathinam & Ngo, Huu-Hao & Wu, Yi-Rui, 2020. "Advanced CRISPR/Cas-based genome editing tools for microbial biofuels production: A review," Renewable Energy, Elsevier, vol. 149(C), pages 1107-1119.

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