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Direct interaction of Plk4 with STIL ensures formation of a single procentriole per parental centriole

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  • Midori Ohta

    (Centrosome Biology Laboratory, Center for Frontier Research, National Institute of Genetics)

  • Tomoko Ashikawa

    (Centrosome Biology Laboratory, Center for Frontier Research, National Institute of Genetics)

  • Yuka Nozaki

    (Centrosome Biology Laboratory, Center for Frontier Research, National Institute of Genetics)

  • Hiroko Kozuka-Hata

    (Medical Proteomics Laboratory, Institute of Medical Science, The University of Tokyo)

  • Hidemasa Goto

    (Aichi Cancer Center Research Institute)

  • Masaki Inagaki

    (Aichi Cancer Center Research Institute)

  • Masaaki Oyama

    (Medical Proteomics Laboratory, Institute of Medical Science, The University of Tokyo)

  • Daiju Kitagawa

    (Centrosome Biology Laboratory, Center for Frontier Research, National Institute of Genetics)

Abstract

Formation of one procentriole next to each pre-existing centriole is essential for centrosome duplication, robust bipolar spindle assembly and maintenance of genome integrity. However, the mechanisms maintaining strict control over centriole copy number are incompletely understood. Here we show that Plk4 and STIL, the key regulators of centriole formation, form a protein complex that provides a scaffold for recruiting HsSAS-6, a major component of the centriolar cartwheel, at the onset of procentriole formation. Furthermore, we demonstrate that phosphorylation of STIL by Plk4 facilitates the STIL/HsSAS-6 interaction and centriolar loading of HsSAS-6. We also provide evidence that negative feedback by centriolar STIL regulates bimodal centriolar distribution of Plk4 and seemingly restricts occurrence of procentriole formation to one site on each parental centriole. Overall, these findings suggest a mechanism whereby coordinated action of three critical factors ensures formation of a single procentriole per parental centriole.

Suggested Citation

  • Midori Ohta & Tomoko Ashikawa & Yuka Nozaki & Hiroko Kozuka-Hata & Hidemasa Goto & Masaki Inagaki & Masaaki Oyama & Daiju Kitagawa, 2014. "Direct interaction of Plk4 with STIL ensures formation of a single procentriole per parental centriole," Nature Communications, Nature, vol. 5(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms6267
    DOI: 10.1038/ncomms6267
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    Cited by:

    1. Jung-Eun Park & Tae-Sung Kim & Yan Zeng & Melissa Mikolaj & Jong Ahn & Muhammad S. Alam & Christina M. Monnie & Victoria Shi & Ming Zhou & Tae-Wook Chun & Frank Maldarelli & Kedar Narayan & Jinwoo Ahn, 2024. "Centrosome amplification and aneuploidy driven by the HIV-1-induced Vpr•VprBP•Plk4 complex in CD4+ T cells," Nature Communications, Nature, vol. 15(1), pages 1-18, December.

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