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Red fluorescent genetically encoded indicator for intracellular hydrogen peroxide

Author

Listed:
  • Yulia G. Ermakova

    (Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry)

  • Dmitry S. Bilan

    (Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry
    NBIC, Moscow Institute of Physics and Technology)

  • Mikhail E. Matlashov

    (Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry
    NBIC, Moscow Institute of Physics and Technology)

  • Natalia M. Mishina

    (Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry)

  • Ksenia N. Markvicheva

    (Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry)

  • Oksana M. Subach

    (NBIC, Moscow Institute of Physics and Technology)

  • Fedor V. Subach

    (NBIC, Moscow Institute of Physics and Technology)

  • Ivan Bogeski

    (School of Medicine, Saarland University)

  • Markus Hoth

    (School of Medicine, Saarland University)

  • Grigori Enikolopov

    (NBIC, Moscow Institute of Physics and Technology
    Cold Spring Harbor Laboratory)

  • Vsevolod V. Belousov

    (Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry
    NBIC, Moscow Institute of Physics and Technology)

Abstract

Reactive oxygen species (ROS) are conserved regulators of numerous cellular functions, and overproduction of ROS is a hallmark of various pathological processes. Genetically encoded fluorescent probes are unique tools to study ROS production in living systems of different scale and complexity. However, the currently available recombinant redox sensors have green emission, which overlaps with the spectra of many other probes. Expanding the spectral range of recombinant in vivo ROS probes would enable multiparametric in vivo ROS detection. Here we present the first genetically encoded red fluorescent sensor for hydrogen peroxide detection, HyPerRed. The performance of this sensor is similar to its green analogues. We demonstrate the utility of the sensor by tracing low concentrations of H2O2 produced in the cytoplasm of cultured cells upon growth factor stimulation. Moreover, using HyPerRed we detect local and transient H2O2 production in the mitochondrial matrix upon inhibition of the endoplasmic reticulum Ca2+ uptake.

Suggested Citation

  • Yulia G. Ermakova & Dmitry S. Bilan & Mikhail E. Matlashov & Natalia M. Mishina & Ksenia N. Markvicheva & Oksana M. Subach & Fedor V. Subach & Ivan Bogeski & Markus Hoth & Grigori Enikolopov & Vsevolo, 2014. "Red fluorescent genetically encoded indicator for intracellular hydrogen peroxide," Nature Communications, Nature, vol. 5(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms6222
    DOI: 10.1038/ncomms6222
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    Cited by:

    1. Pamela E. Molinari & Adriana R. Krapp & Andrea Weiner & Hannes M. Beyer & Arun Kumar Kondadi & Tim Blomeier & Melina López & Pilar Bustos-Sanmamed & Evelyn Tevere & Wilfried Weber & Andreas S. Reicher, 2023. "NERNST: a genetically-encoded ratiometric non-destructive sensing tool to estimate NADP(H) redox status in bacterial, plant and animal systems," Nature Communications, Nature, vol. 14(1), pages 1-18, December.

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