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Suppression of the FOXM1 transcriptional programme via novel small molecule inhibition

Author

Listed:
  • Michael V. Gormally

    (University Chemical Laboratory, University of Cambridge
    Cancer Research UK, Li Ka Shing Centre, Cambridge Institute
    National Center for Advancing Translational Sciences, NIH)

  • Thomas S. Dexheimer

    (National Center for Advancing Translational Sciences, NIH)

  • Giovanni Marsico

    (Cancer Research UK, Li Ka Shing Centre, Cambridge Institute)

  • Deborah A. Sanders

    (Cancer Research UK, Li Ka Shing Centre, Cambridge Institute)

  • Christopher Lowe

    (University Chemical Laboratory, University of Cambridge)

  • Dijana Matak-Vinković

    (University Chemical Laboratory, University of Cambridge)

  • Sam Michael

    (National Center for Advancing Translational Sciences, NIH)

  • Ajit Jadhav

    (National Center for Advancing Translational Sciences, NIH)

  • Ganesha Rai

    (National Center for Advancing Translational Sciences, NIH)

  • David J. Maloney

    (National Center for Advancing Translational Sciences, NIH)

  • Anton Simeonov

    (National Center for Advancing Translational Sciences, NIH)

  • Shankar Balasubramanian

    (University Chemical Laboratory, University of Cambridge
    Cancer Research UK, Li Ka Shing Centre, Cambridge Institute)

Abstract

The transcription factor FOXM1 binds to sequence-specific motifs on DNA (C/TAAACA) through its DNA-binding domain (DBD) and activates proliferation- and differentiation-associated genes. Aberrant overexpression of FOXM1 is a key feature in oncogenesis and progression of many human cancers. Here—from a high-throughput screen applied to a library of 54,211 small molecules—we identify novel small molecule inhibitors of FOXM1 that block DNA binding. One of the identified compounds, FDI-6 (NCGC00099374), is characterized in depth and is shown to bind directly to FOXM1 protein, to displace FOXM1 from genomic targets in MCF-7 breast cancer cells, and induce concomitant transcriptional downregulation. Global transcript profiling of MCF-7 cells by RNA-seq shows that FDI-6 specifically downregulates FOXM1-activated genes with FOXM1 occupancy confirmed by ChIP-PCR. This small molecule-mediated effect is selective for FOXM1-controlled genes with no effect on genes regulated by homologous forkhead family factors.

Suggested Citation

  • Michael V. Gormally & Thomas S. Dexheimer & Giovanni Marsico & Deborah A. Sanders & Christopher Lowe & Dijana Matak-Vinković & Sam Michael & Ajit Jadhav & Ganesha Rai & David J. Maloney & Anton Simeon, 2014. "Suppression of the FOXM1 transcriptional programme via novel small molecule inhibition," Nature Communications, Nature, vol. 5(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms6165
    DOI: 10.1038/ncomms6165
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    Cited by:

    1. Hue M. La & Jinyue Liao & Julien M. D. Legrand & Fernando J. Rossello & Ai-Leen Chan & Vijesh Vaghjiani & Jason E. Cain & Antonella Papa & Tin Lap Lee & Robin M. Hobbs, 2022. "Distinctive molecular features of regenerative stem cells in the damaged male germline," Nature Communications, Nature, vol. 13(1), pages 1-17, December.

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