IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v5y2014i1d10.1038_ncomms6074.html
   My bibliography  Save this article

Fucose-based PAMPs prime dendritic cells for follicular T helper cell polarization via DC-SIGN-dependent IL-27 production

Author

Listed:
  • Sonja I. Gringhuis

    (Academic Medical Center, University of Amsterdam)

  • Tanja M. Kaptein

    (Academic Medical Center, University of Amsterdam)

  • Brigitte A. Wevers

    (Academic Medical Center, University of Amsterdam)

  • Michiel van der Vlist

    (Academic Medical Center, University of Amsterdam)

  • Elsenoor J. Klaver

    (VU University Medical Center)

  • Irma van Die

    (VU University Medical Center)

  • Lianne E. M. Vriend

    (Academic Medical Center, University of Amsterdam)

  • Marein A. W. P. de Jong

    (Academic Medical Center, University of Amsterdam)

  • Teunis B. H. Geijtenbeek

    (Academic Medical Center, University of Amsterdam)

Abstract

Dendritic cells (DCs) orchestrate antibody-mediated responses to combat extracellular pathogens including parasites by initiating T helper cell differentiation. Here we demonstrate that carbohydrate-specific signalling by DC-SIGN drives follicular T helper cell (TFH) differentiation via IL-27 expression. Fucose, but not mannose, engagement of DC-SIGN results in activation of IKKε, which collaborates with type I IFNR signalling to induce formation and activation of transcription factor ISGF3. Notably, ISGF3 induces expression of IL-27 subunit p28, and subsequent IL-27 secreted by DC-SIGN-primed DCs is pivotal for the induction of Bcl-6+CXCR5+PD-1hiFoxp1lo TFH cells, IL-21 secretion by TFH cells and T-cell-dependent IgG production by B cells. Thus, we have identified an essential role for DC-SIGN-induced ISGF3 by fucose-based PAMPs in driving IL-27 and subsequent TFH polarization, which might be harnessed for vaccination design.

Suggested Citation

  • Sonja I. Gringhuis & Tanja M. Kaptein & Brigitte A. Wevers & Michiel van der Vlist & Elsenoor J. Klaver & Irma van Die & Lianne E. M. Vriend & Marein A. W. P. de Jong & Teunis B. H. Geijtenbeek, 2014. "Fucose-based PAMPs prime dendritic cells for follicular T helper cell polarization via DC-SIGN-dependent IL-27 production," Nature Communications, Nature, vol. 5(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms6074
    DOI: 10.1038/ncomms6074
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/ncomms6074
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/ncomms6074?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Alexiane Decout & Ioannis Krasias & Lauren Roberts & Belen Gimeno Molina & Chloé Charenton & Daniel Brown Romero & Qiong Y. Tee & Julian R. Marchesi & Sherrianne Ng & Lynne Sykes & Phillip R. Bennett , 2024. "Lactobacillus crispatus S-layer proteins modulate innate immune response and inflammation in the lower female reproductive tract," Nature Communications, Nature, vol. 15(1), pages 1-12, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms6074. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.