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Determination of collagen fibril size via absolute measurements of second-harmonic generation signals

Author

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  • Stéphane Bancelin

    (Laboratoire d’Optique et Biosciences, Ecole Polytechnique, CNRS, INSERM U696, Route de Saclay)

  • Carole Aimé

    (Sorbonne Universités, UPMC Univ Paris 06, CNRS, Laboratoire de Chimie de la Matière Condensée de Paris, Collège de France)

  • Ivan Gusachenko

    (Laboratoire d’Optique et Biosciences, Ecole Polytechnique, CNRS, INSERM U696, Route de Saclay)

  • Laura Kowalczuk

    (Sorbonne Universités, UPMC Univ Paris 06, INSERM UMRS 872, Team17: Physiopathology of Ocular Diseases, Therapeutic Innovations, Centre de Recherche des Cordeliers)

  • Gaël Latour

    (Laboratoire d’Optique et Biosciences, Ecole Polytechnique, CNRS, INSERM U696, Route de Saclay)

  • Thibaud Coradin

    (Sorbonne Universités, UPMC Univ Paris 06, CNRS, Laboratoire de Chimie de la Matière Condensée de Paris, Collège de France)

  • Marie-Claire Schanne-Klein

    (Laboratoire d’Optique et Biosciences, Ecole Polytechnique, CNRS, INSERM U696, Route de Saclay)

Abstract

The quantification of collagen fibril size is a major issue for the investigation of pathological disorders associated with structural defects of the extracellular matrix. Second-harmonic generation microscopy is a powerful technique to characterize the macromolecular organization of collagen in unstained biological tissues. Nevertheless, due to the complex coherent building of this nonlinear optical signal, it has never been used to measure fibril diameter so far. Here we report absolute measurements of second-harmonic signals from isolated fibrils down to 30 nm diameter, via implementation of correlative second-harmonic-electron microscopy. Moreover, using analytical and numerical calculations, we demonstrate that the high sensitivity of this technique originates from the parallel alignment of collagen triple helices within fibrils and the subsequent constructive interferences of second-harmonic radiations. Finally, we use these absolute measurements as a calibration for ex vivo quantification of fibril diameter in the Descemet’s membrane of a diabetic rat cornea.

Suggested Citation

  • Stéphane Bancelin & Carole Aimé & Ivan Gusachenko & Laura Kowalczuk & Gaël Latour & Thibaud Coradin & Marie-Claire Schanne-Klein, 2014. "Determination of collagen fibril size via absolute measurements of second-harmonic generation signals," Nature Communications, Nature, vol. 5(1), pages 1-8, December.
    • Handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms5920
    DOI: 10.1038/ncomms5920
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    Cited by:

    1. Katrein Sauer & Ivo Zizak & Jean-Baptiste Forien & Alexander Rack & Ernesto Scoppola & Paul Zaslansky, 2022. "Primary radiation damage in bone evolves via collagen destruction by photoelectrons and secondary emission self-absorption," Nature Communications, Nature, vol. 13(1), pages 1-12, December.

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