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Deciphering Fur transcriptional regulatory network highlights its complex role beyond iron metabolism in Escherichia coli

Author

Listed:
  • Sang Woo Seo

    (University of California San Diego)

  • Donghyuk Kim

    (University of California San Diego)

  • Haythem Latif

    (University of California San Diego)

  • Edward J. O’Brien

    (University of California San Diego)

  • Richard Szubin

    (University of California San Diego)

  • Bernhard O. Palsson

    (University of California San Diego
    Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark)

Abstract

The ferric uptake regulator (Fur) plays a critical role in the transcriptional regulation of iron metabolism. However, the full regulatory potential of Fur remains undefined. Here we comprehensively reconstruct the Fur transcriptional regulatory network in Escherichia coli K-12 MG1655 in response to iron availability using genome-wide measurements. Integrative data analysis reveals that a total of 81 genes in 42 transcription units are directly regulated by three different modes of Fur regulation, including apo- and holo-Fur activation and holo-Fur repression. We show that Fur connects iron transport and utilization enzymes with negative-feedback loop pairs for iron homeostasis. In addition, direct involvement of Fur in the regulation of DNA synthesis, energy metabolism and biofilm development is found. These results show how Fur exhibits a comprehensive regulatory role affecting many fundamental cellular processes linked to iron metabolism in order to coordinate the overall response of E. coli to iron availability.

Suggested Citation

  • Sang Woo Seo & Donghyuk Kim & Haythem Latif & Edward J. O’Brien & Richard Szubin & Bernhard O. Palsson, 2014. "Deciphering Fur transcriptional regulatory network highlights its complex role beyond iron metabolism in Escherichia coli," Nature Communications, Nature, vol. 5(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms5910
    DOI: 10.1038/ncomms5910
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    Cited by:

    1. Yichao Han & Wanji Li & Alden Filko & Jingyao Li & Fuzhong Zhang, 2023. "Genome-wide promoter responses to CRISPR perturbations of regulators reveal regulatory networks in Escherichia coli," Nature Communications, Nature, vol. 14(1), pages 1-13, December.

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