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Basal foot MTOC organizes pillar MTs required for coordination of beating cilia

Author

Listed:
  • Daniel K. Clare

    (Institute of Structural and Molecular Biology, Birkbeck College and University College of London, Malet Street)

  • Jérémy Magescas

    (Morphogenesis, homeostasis and pathologies, Jacques Monod Institute, CNRS-UMR7592, Paris Diderot University, 15 Rue Hélène Brion)

  • Tristan Piolot

    (ImagoSeine, Jacques Monod Institute, CNRS-UMR7592, Paris Diderot University, 15 Rue Hélène Brion, 75205
    Present address: PICT-IBiSA, CNRS-UMR 3215 Developmental Biology Building, 26 Rue d’Ulm Curie Institute, 75248 Paris, France)

  • Maud Dumoux

    (Institute of Structural and Molecular Biology, Birkbeck College and University College of London, Malet Street)

  • Christine Vesque

    (Laboratoire de Biologie du Développement, INSERM U969-UMR 7622, Pierre and Marie Curie University (UPMC), 9 Quai Saint-Bernard)

  • Evelyne Pichard

    (Morphogenesis, homeostasis and pathologies, Jacques Monod Institute, CNRS-UMR7592, Paris Diderot University, 15 Rue Hélène Brion)

  • Tien Dang

    (Morphogenesis, homeostasis and pathologies, Jacques Monod Institute, CNRS-UMR7592, Paris Diderot University, 15 Rue Hélène Brion)

  • Boris Duvauchelle

    (Morphogenesis, homeostasis and pathologies, Jacques Monod Institute, CNRS-UMR7592, Paris Diderot University, 15 Rue Hélène Brion)

  • Françoise Poirier

    (Morphogenesis, homeostasis and pathologies, Jacques Monod Institute, CNRS-UMR7592, Paris Diderot University, 15 Rue Hélène Brion)

  • Delphine Delacour

    (Morphogenesis, homeostasis and pathologies, Jacques Monod Institute, CNRS-UMR7592, Paris Diderot University, 15 Rue Hélène Brion)

Abstract

Coordination of ciliary beating is essential to ensure mucus clearance in the airway tract. The orientation and synchronization of ciliary motion responds in part to the organization of the underlying cytoskeletal networks. Using electron tomography on mouse trachea, we show that basal bodies are collectively hooked at the cortex by a regular microtubule array composed of 4–5 microtubules. Removal of galectin-3, one of basal-body components, provokes misrecruitment of γ-tubulin, disorganization of this microtubule framework emanating from the basal-foot cap, together with loss of basal-body alignment and cilium orientation, defects in cilium organization and reduced fluid flow in the tracheal lumen. We conclude that galectin-3 plays a crucial role in the maintenance of the microtubule-organizing centre of the cilium and the ‘pillar’ microtubules, and that this network is instrumental for the coordinated orientation and stabilization of motile cilia.

Suggested Citation

  • Daniel K. Clare & Jérémy Magescas & Tristan Piolot & Maud Dumoux & Christine Vesque & Evelyne Pichard & Tien Dang & Boris Duvauchelle & Françoise Poirier & Delphine Delacour, 2014. "Basal foot MTOC organizes pillar MTs required for coordination of beating cilia," Nature Communications, Nature, vol. 5(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms5888
    DOI: 10.1038/ncomms5888
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    Cited by:

    1. Toshinori Namba & Shuji Ishihara, 2020. "Cytoskeleton polarity is essential in determining orientational order in basal bodies of multi-ciliated cells," PLOS Computational Biology, Public Library of Science, vol. 16(2), pages 1-18, February.

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