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G1-arrested newborn cells are the predominant infectious form of the pathogen Brucella abortus

Author

Listed:
  • Michaël Deghelt

    (Microorganisms biology research unit (URBM), University of Namur (UNamur))

  • Caroline Mullier

    (Microorganisms biology research unit (URBM), University of Namur (UNamur))

  • Jean-François Sternon

    (Microorganisms biology research unit (URBM), University of Namur (UNamur))

  • Nayla Francis

    (Microorganisms biology research unit (URBM), University of Namur (UNamur))

  • Géraldine Laloux

    (Cellular and Developmental Biology, Yale University
    Howard Hughes Medical Institute, Yale University
    Yale School of Medicine)

  • Delphine Dotreppe

    (Microorganisms biology research unit (URBM), University of Namur (UNamur))

  • Charles Van der Henst

    (Microorganisms biology research unit (URBM), University of Namur (UNamur))

  • Christine Jacobs-Wagner

    (Cellular and Developmental Biology, Yale University
    Howard Hughes Medical Institute, Yale University
    Yale School of Medicine)

  • Jean-Jacques Letesson

    (Microorganisms biology research unit (URBM), University of Namur (UNamur))

  • Xavier De Bolle

    (Microorganisms biology research unit (URBM), University of Namur (UNamur))

Abstract

Several intracellular pathogens, such as Brucella abortus, display a biphasic infection process starting with a non-proliferative stage of unclear nature. Here, we study the cell cycle of B. abortus at the single-cell level, in culture and during infection of HeLa cells and macrophages. The localization of segregation and replication loci of the two bacterial chromosomes indicates that, immediately after being engulfed by host-cell endocytic vacuoles, most bacterial cells are newborn. These bacterial cells do not initiate DNA replication for the next 4 to 6 h, indicating a G1 arrest. Moreover, growth is completely stopped during that time, reflecting a global cell cycle block. Growth and DNA replication resume later, although bacteria still reside within endosomal-like compartments. We hypothesize that the predominance of G1-arrested bacteria in the infectious population, and the bacterial cell cycle arrest following internalization, may constitute a widespread strategy among intracellular pathogens to colonize new proliferation niches.

Suggested Citation

  • Michaël Deghelt & Caroline Mullier & Jean-François Sternon & Nayla Francis & Géraldine Laloux & Delphine Dotreppe & Charles Van der Henst & Christine Jacobs-Wagner & Jean-Jacques Letesson & Xavier De , 2014. "G1-arrested newborn cells are the predominant infectious form of the pathogen Brucella abortus," Nature Communications, Nature, vol. 5(1), pages 1-12, September.
    • Handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms5366
    DOI: 10.1038/ncomms5366
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    Cited by:

    1. Caroline Servais & Victoria Vassen & Audrey Verhaeghe & Nina Küster & Elodie Carlier & Léa Phégnon & Aurélie Mayard & Nicolas Auberger & Stéphane Vincent & Xavier De Bolle, 2023. "Lipopolysaccharide biosynthesis and traffic in the envelope of the pathogen Brucella abortus," Nature Communications, Nature, vol. 14(1), pages 1-13, December.
    2. Théophile Niault & Ariel Talavera & Eric Le Cam & Sonia Baconnais & Ole Skovgaard & Florian Fournes & Léa Wagner & Hedvig Tamman & Andrew Thompson & Dannele Echemendia-Blanco & Noa Guzzi & Abel Garcia, 2025. "Dynamic transitions of initiator binding coordinate the replication of the two chromosomes in Vibrio cholerae," Nature Communications, Nature, vol. 16(1), pages 1-16, December.

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