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The fidelity of the ligation step determines how ends are resolved during nonhomologous end joining

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  • Crystal A. Waters

    (Lineberger Comprehensive Cancer Center, University of North Carolina)

  • Natasha T. Strande

    (Lineberger Comprehensive Cancer Center, University of North Carolina)

  • John M. Pryor

    (Lineberger Comprehensive Cancer Center, University of North Carolina)

  • Christina N. Strom

    (Lineberger Comprehensive Cancer Center, University of North Carolina)

  • Piotr Mieczkowski

    (University of North Carolina)

  • Martin D. Burkhalter

    (Lineberger Comprehensive Cancer Center, University of North Carolina)

  • Sehyun Oh

    (Molecular Biology, and Biophysics, University of Minnosota, 6-155 Jackson Hall, 321 Church Street SE)

  • Bahjat F. Qaqish

    (Biostatistics core, Lineberger Comprehensive Cancer Center, University of North Carolina)

  • Dominic T. Moore

    (Biostatistics core, Lineberger Comprehensive Cancer Center, University of North Carolina)

  • Eric A. Hendrickson

    (Molecular Biology, and Biophysics, University of Minnosota, 6-155 Jackson Hall, 321 Church Street SE)

  • Dale A. Ramsden

    (Lineberger Comprehensive Cancer Center, University of North Carolina)

Abstract

Nonhomologous end joining (NHEJ) can effectively resolve chromosome breaks despite diverse end structures; however, it is unclear how the steps employed for resolution are determined. We sought to address this question by analysing cellular NHEJ of ends with systematically mispaired and damaged termini. We show NHEJ is uniquely proficient at bypassing subtle terminal mispairs and radiomimetic damage by direct ligation. Nevertheless, bypass ability varies widely, with increases in mispair severity gradually reducing bypass products from 85 to 6%. End-processing by nucleases and polymerases is increased to compensate, although paths with the fewest number of steps to generate a substrate suitable for ligation are favoured. Thus, both the frequency and nature of end processing are tailored to meet the needs of the ligation step. We propose a model where the ligase organizes all steps during NHEJ within the stable paired-end complex to limit end processing and associated errors.

Suggested Citation

  • Crystal A. Waters & Natasha T. Strande & John M. Pryor & Christina N. Strom & Piotr Mieczkowski & Martin D. Burkhalter & Sehyun Oh & Bahjat F. Qaqish & Dominic T. Moore & Eric A. Hendrickson & Dale A., 2014. "The fidelity of the ligation step determines how ends are resolved during nonhomologous end joining," Nature Communications, Nature, vol. 5(1), pages 1-11, September.
    • Handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms5286
    DOI: 10.1038/ncomms5286
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    Cited by:

    1. Benjamin M. Stinson & Sean M. Carney & Johannes C. Walter & Joseph J. Loparo, 2024. "Structural role for DNA Ligase IV in promoting the fidelity of non-homologous end joining," Nature Communications, Nature, vol. 15(1), pages 1-13, December.

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