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Identification of platelet function defects by multi-parameter assessment of thrombus formation

Author

Listed:
  • Susanne M. de Witt

    (Cardiovascular Research Institute Maastricht (CARIM), Maastricht University)

  • Frauke Swieringa

    (Cardiovascular Research Institute Maastricht (CARIM), Maastricht University)

  • Rachel Cavill

    (Maastricht University)

  • Moniek M. E. Lamers

    (Cardiovascular Research Institute Maastricht (CARIM), Maastricht University)

  • Roger van Kruchten

    (Cardiovascular Research Institute Maastricht (CARIM), Maastricht University)

  • Tom Mastenbroek

    (Cardiovascular Research Institute Maastricht (CARIM), Maastricht University)

  • Constance Baaten

    (Cardiovascular Research Institute Maastricht (CARIM), Maastricht University)

  • Susan Coort

    (Maastricht University)

  • Nicholas Pugh

    (Anglia Ruskin University)

  • Ansgar Schulz

    (Pediatric Centre, University Clinic Ulm, Universitätsklinik Für Kinder- und Jugendmedizin)

  • Inge Scharrer

    (Center for Thrombosis and Haemostasis (CTH), Universitätsmedizin der Johannes Gutenberg, University of Mainz)

  • Kerstin Jurk

    (Center for Thrombosis and Haemostasis (CTH), Universitätsmedizin der Johannes Gutenberg, University of Mainz)

  • Barbara Zieger

    (University Medical Centre Freiburg)

  • Kenneth J. Clemetson

    (Inselspital, University of Bern)

  • Richard W. Farndale

    (University of Cambridge)

  • Johan W. M. Heemskerk

    (Cardiovascular Research Institute Maastricht (CARIM), Maastricht University)

  • Judith M.E.M. Cosemans

    (Cardiovascular Research Institute Maastricht (CARIM), Maastricht University)

Abstract

Assays measuring platelet aggregation (thrombus formation) at arterial shear rate mostly use collagen as only platelet-adhesive surface. Here we report a multi-surface and multi-parameter flow assay to characterize thrombus formation in whole blood from healthy subjects and patients with platelet function deficiencies. A systematic comparison is made of 52 adhesive surfaces with components activating the main platelet-adhesive receptors, and of eight output parameters reflecting distinct stages of thrombus formation. Three types of thrombus formation can be identified with a predicted hierarchy of the following receptors: glycoprotein (GP)VI, C-type lectin-like receptor-2 (CLEC-2)>GPIb>α6β1, αIIbβ3>α2β1>CD36, α5β1, αvβ3. Application with patient blood reveals distinct abnormalities in thrombus formation in patients with severe combined immune deficiency, Glanzmann’s thrombasthenia, Hermansky–Pudlak syndrome, May–Hegglin anomaly or grey platelet syndrome. We suggest this test may be useful for the diagnosis of patients with suspected bleeding disorders or a pro-thrombotic tendency.

Suggested Citation

  • Susanne M. de Witt & Frauke Swieringa & Rachel Cavill & Moniek M. E. Lamers & Roger van Kruchten & Tom Mastenbroek & Constance Baaten & Susan Coort & Nicholas Pugh & Ansgar Schulz & Inge Scharrer & Ke, 2014. "Identification of platelet function defects by multi-parameter assessment of thrombus formation," Nature Communications, Nature, vol. 5(1), pages 1-13, September.
    • Handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms5257
    DOI: 10.1038/ncomms5257
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    1. “Jimmy” Xu, Zhenning & Ramirez, Edward & Liu, Pan & Frankwick, Gary L., 2024. "Evaluating underlying factor structures using novel machine learning algorithms: An empirical and simulation study," Journal of Business Research, Elsevier, vol. 173(C).

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