IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v5y2014i1d10.1038_ncomms5226.html
   My bibliography  Save this article

Lineage-restricted function of the pluripotency factor NANOG in stratified epithelia

Author

Listed:
  • Daniela Piazzolla

    (Tumour Suppression Group, Spanish National Cancer Research Centre (CNIO))

  • Adelaida R. Palla

    (Tumour Suppression Group, Spanish National Cancer Research Centre (CNIO))

  • Cristina Pantoja

    (Tumour Suppression Group, Spanish National Cancer Research Centre (CNIO))

  • Marta Cañamero

    (Histopathology Core Unit, CNIO)

  • Ignacio Perez de Castro

    (CNIO)

  • Sagrario Ortega

    (Transgenic Mice Core Unit, CNIO)

  • Gonzalo Gómez-López

    (Bioinformatics Unit, CNIO)

  • Orlando Dominguez

    (Genomics Core Unit, CNIO)

  • Diego Megías

    (Confocal Microscopy Core Unit, CNIO)

  • Giovanna Roncador

    (Monoclonal Antibodies Core Unit, Spanish National Cancer Research Centre (CNIO))

  • Jose L. Luque-Garcia

    (Faculty of Chemistry, Complutense University of Madrid)

  • Beatriz Fernandez-Tresguerres

    (Centro Nacional de Investigaciones Cardiovasculares (CNIC))

  • Agustin F. Fernandez

    (Cancer Epigenetics Laboratory, Instituto Universitario de Oncología del Principado de Asturias (IUOPA), HUCA, Universidad de Oviedo)

  • Mario F. Fraga

    (Cancer Epigenetics Laboratory, Instituto Universitario de Oncología del Principado de Asturias (IUOPA), HUCA, Universidad de Oviedo
    Centro Nacional de Biotecnología/CNB-CSIC)

  • Manuel Rodriguez-Justo

    (University College Hospital)

  • Miguel Manzanares

    (Centro Nacional de Investigaciones Cardiovasculares (CNIC))

  • Marta Sánchez-Carbayo

    (CIC bioGUNE, Bizkaia Technology Park, Derio 48160 and Ikerbasque, Basque Foundation for Science)

  • Juana María García-Pedrero

    (Hospital Universitario Central de Asturias and Instituto Universitario de Oncología del Principado de Asturias (IUOPA), Universidad de Oviedo)

  • Juan P. Rodrigo

    (Hospital Universitario Central de Asturias and Instituto Universitario de Oncología del Principado de Asturias (IUOPA), Universidad de Oviedo)

  • Marcos Malumbres

    (CNIO)

  • Manuel Serrano

    (Tumour Suppression Group, Spanish National Cancer Research Centre (CNIO))

Abstract

NANOG is a pluripotency transcription factor in embryonic stem cells; however, its role in adult tissues remains largely unexplored. Here we show that mouse NANOG is selectively expressed in stratified epithelia, most notably in the oesophagus where the Nanog promoter is hypomethylated. Interestingly, inducible ubiquitous overexpression of NANOG in mice causes hyperplasia selectively in the oesophagus, in association with increased cell proliferation. NANOG transcriptionally activates the mitotic programme, including Aurora A kinase (Aurka), in stratified epithelia, and endogenous NANOG directly binds to the Aurka promoter in primary keratinocytes. Interestingly, overexpression of Nanog or Aurka in mice increased proliferation and aneuploidy in the oesophageal basal epithelium. Finally, inactivation of NANOG in cell lines from oesophageal or head and neck squamous cell carcinomas (ESCCs or HNSCCs, respectively) results in lower levels of AURKA and decreased proliferation, and NANOG and AURKA expression are positively correlated in HNSCCs. Together, these results indicate that NANOG has a lineage-restricted mitogenic function in stratified epithelia.

Suggested Citation

  • Daniela Piazzolla & Adelaida R. Palla & Cristina Pantoja & Marta Cañamero & Ignacio Perez de Castro & Sagrario Ortega & Gonzalo Gómez-López & Orlando Dominguez & Diego Megías & Giovanna Roncador & Jos, 2014. "Lineage-restricted function of the pluripotency factor NANOG in stratified epithelia," Nature Communications, Nature, vol. 5(1), pages 1-14, September.
    • Handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms5226
    DOI: 10.1038/ncomms5226
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/ncomms5226
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/ncomms5226?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Nika Rajabian & Izuagie Ikhapoh & Shahryar Shahini & Debanik Choudhury & Ramkumar Thiyagarajan & Aref Shahini & Joseph Kulczyk & Kendall Breed & Shilpashree Saha & Mohamed Alaa Mohamed & Susan B. Udin, 2023. "Methionine adenosyltransferase2A inhibition restores metabolism to improve regenerative capacity and strength of aged skeletal muscle," Nature Communications, Nature, vol. 14(1), pages 1-19, December.
    2. Naveen Kumar & Pon Ganish Prakash & Christian Wentland & Shilpa Mary Kurian & Gaurav Jethva & Volker Brinkmann & Hans-Joachim Mollenkopf & Tobias Krammer & Christophe Toussaint & Antoine-Emmanuel Sali, 2024. "Decoding spatiotemporal transcriptional dynamics and epithelial fibroblast crosstalk during gastroesophageal junction development through single cell analysis," Nature Communications, Nature, vol. 15(1), pages 1-20, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms5226. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.