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Light-inducible receptor tyrosine kinases that regulate neurotrophin signalling

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  • Ki-Young Chang

    (Center for Cognition and Sociality, Institute for Basic Science (IBS)
    Korea Advanced Institute of Science and Technology (KAIST))

  • Doyeon Woo

    (Korea Advanced Institute of Science and Technology (KAIST)
    WCI Center for Functional Connectomics, Korea Institute of Science and Technology (KIST))

  • Hyunjin Jung

    (Korea Advanced Institute of Science and Technology (KAIST))

  • Sangkyu Lee

    (Center for Cognition and Sociality, Institute for Basic Science (IBS)
    Korea Advanced Institute of Science and Technology (KAIST))

  • Sungsoo Kim

    (Korea Advanced Institute of Science and Technology (KAIST))

  • Joungha Won

    (Korea Advanced Institute of Science and Technology (KAIST))

  • Taeyoon Kyung

    (Korea Advanced Institute of Science and Technology (KAIST))

  • Hyerim Park

    (Korea Advanced Institute of Science and Technology (KAIST))

  • Nury Kim

    (Center for Cognition and Sociality, Institute for Basic Science (IBS)
    Korea Advanced Institute of Science and Technology (KAIST))

  • Hee Won Yang

    (Korea Advanced Institute of Science and Technology (KAIST))

  • Jae-Yong Park

    (WCI Center for Functional Connectomics, Korea Institute of Science and Technology (KIST)
    Institute of Health Science, and Medical Research Center for Neural Dysfunction, Gyeongsang National University School of Medicine)

  • Eun Mi Hwang

    (WCI Center for Functional Connectomics, Korea Institute of Science and Technology (KIST))

  • Daesoo Kim

    (Korea Advanced Institute of Science and Technology (KAIST))

  • Won Do Heo

    (Center for Cognition and Sociality, Institute for Basic Science (IBS)
    Korea Advanced Institute of Science and Technology (KAIST))

Abstract

Receptor tyrosine kinases (RTKs) are a family of cell-surface receptors that have a key role in regulating critical cellular processes. Here, to understand and precisely control RTK signalling, we report the development of a genetically encoded, photoactivatable Trk (tropomyosin-related kinase) family of RTKs using a light-responsive module based on Arabidopsis thaliana cryptochrome 2. Blue-light stimulation (488 nm) of mammalian cells harbouring these receptors robustly upregulates canonical Trk signalling. A single light stimulus triggers transient signalling activation, which is reversibly tuned by repetitive delivery of blue-light pulses. In addition, the light-provoked process is induced in a spatially restricted and cell-specific manner. A prolonged patterned illumination causes sustained activation of extracellular signal-regulated kinase and promotes neurite outgrowth in a neuronal cell line, and induces filopodia formation in rat hippocampal neurons. These light-controllable receptors are expected to create experimental opportunities to spatiotemporally manipulate many biological processes both in vitro and in vivo.

Suggested Citation

  • Ki-Young Chang & Doyeon Woo & Hyunjin Jung & Sangkyu Lee & Sungsoo Kim & Joungha Won & Taeyoon Kyung & Hyerim Park & Nury Kim & Hee Won Yang & Jae-Yong Park & Eun Mi Hwang & Daesoo Kim & Won Do Heo, 2014. "Light-inducible receptor tyrosine kinases that regulate neurotrophin signalling," Nature Communications, Nature, vol. 5(1), pages 1-10, September.
    • Handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms5057
    DOI: 10.1038/ncomms5057
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    Cited by:

    1. Dennis Vettkötter & Martin Schneider & Brady D. Goulden & Holger Dill & Jana Liewald & Sandra Zeiler & Julia Guldan & Yilmaz Arda Ateş & Shigeki Watanabe & Alexander Gottschalk, 2022. "Rapid and reversible optogenetic silencing of synaptic transmission by clustering of synaptic vesicles," Nature Communications, Nature, vol. 13(1), pages 1-17, December.
    2. Wenping Zhou & Wenxue Li & Shisheng Wang & Barbora Salovska & Zhenyi Hu & Bo Tao & Yi Di & Ujwal Punyamurtula & Benjamin E. Turk & William C. Sessa & Yansheng Liu, 2023. "An optogenetic-phosphoproteomic study reveals dynamic Akt1 signaling profiles in endothelial cells," Nature Communications, Nature, vol. 14(1), pages 1-17, December.
    3. Rodrigo G. Fernandez Lahore & Niccolò P. Pampaloni & Enrico Schiewer & M.-Marcel Heim & Linda Tillert & Johannes Vierock & Johannes Oppermann & Jakob Walther & Dietmar Schmitz & David Owald & Andrew J, 2022. "Calcium-permeable channelrhodopsins for the photocontrol of calcium signalling," Nature Communications, Nature, vol. 13(1), pages 1-18, December.

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