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Targeted genomic rearrangements using CRISPR/Cas technology

Author

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  • Peter S. Choi

    (Dana-Farber Cancer Institute
    Broad Institute of MIT and Harvard, Cancer Program)

  • Matthew Meyerson

    (Dana-Farber Cancer Institute
    Broad Institute of MIT and Harvard, Cancer Program
    Harvard Medical School)

Abstract

Genomic rearrangements are frequently observed in cancer cells but have been difficult to generate in a highly specific manner for functional analysis. Here we report the application of CRISPR/Cas technology to successfully generate several types of chromosomal rearrangements implicated as driver events in lung cancer, including the CD74-ROS1 translocation event and the EML4-ALK and KIF5B-RET inversion events. Our results demonstrate that Cas9-induced DNA breaks promote efficient rearrangement between pairs of targeted loci, providing a highly tractable approach for the study of genomic rearrangements.

Suggested Citation

  • Peter S. Choi & Matthew Meyerson, 2014. "Targeted genomic rearrangements using CRISPR/Cas technology," Nature Communications, Nature, vol. 5(1), pages 1-6, September.
    • Handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms4728
    DOI: 10.1038/ncomms4728
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    1. Siobhan Rice & Thomas Jackson & Nicholas T. Crump & Nicholas Fordham & Natalina Elliott & Sorcha O’Byrne & Maria del Mar Lara Fanego & Dilys Addy & Trisevgeni Crabb & Carryl Dryden & Sarah Inglott & D, 2021. "A human fetal liver-derived infant MLL-AF4 acute lymphoblastic leukemia model reveals a distinct fetal gene expression program," Nature Communications, Nature, vol. 12(1), pages 1-13, December.
    2. Taek-Chin Cheong & Ahram Jang & Qi Wang & Giulia C. Leonardi & Biagio Ricciuti & Joao V. Alessi & Alessandro Di Federico & Mark M. Awad & Maria K. Lehtinen & Marian H. Harris & Roberto Chiarle, 2024. "Mechanistic patterns and clinical implications of oncogenic tyrosine kinase fusions in human cancers," Nature Communications, Nature, vol. 15(1), pages 1-19, December.

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