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Correlated optical and isotopic nanoscopy

Author

Listed:
  • Sinem K. Saka

    (University of Göttingen Medical Centre, and Centre for Nanoscale Microscopy and Molecular Physiology of the Brain (CNMPB)
    International Max Planck Research School)

  • Angela Vogts

    (Leibniz-Institute for Baltic Sea Research)

  • Katharina Kröhnert

    (University of Göttingen Medical Centre, and Centre for Nanoscale Microscopy and Molecular Physiology of the Brain (CNMPB))

  • François Hillion

    (Cameca, 29 Quai des Grésillons)

  • Silvio O Rizzoli

    (University of Göttingen Medical Centre, and Centre for Nanoscale Microscopy and Molecular Physiology of the Brain (CNMPB))

  • Johannes T. Wessels

    (University of Göttingen Medical Center)

Abstract

The isotopic composition of different materials can be imaged by secondary ion mass spectrometry. In biology, this method is mainly used to study cellular metabolism and turnover, by pulsing the cells with marker molecules such as amino acids labelled with stable isotopes (15N, 13C). The incorporation of the markers is then imaged with a lateral resolution that can surpass 100 nm. However, secondary ion mass spectrometry cannot identify specific subcellular structures like organelles, and needs to be correlated with a second technique, such as fluorescence imaging. Here, we present a method based on stimulated emission depletion microscopy that provides correlated optical and isotopic nanoscopy (COIN) images. We use this approach to study the protein turnover in different organelles from cultured hippocampal neurons. Correlated optical and isotopic nanoscopy can be applied to a variety of biological samples, and should therefore enable the investigation of the isotopic composition of many organelles and subcellular structures.

Suggested Citation

  • Sinem K. Saka & Angela Vogts & Katharina Kröhnert & François Hillion & Silvio O Rizzoli & Johannes T. Wessels, 2014. "Correlated optical and isotopic nanoscopy," Nature Communications, Nature, vol. 5(1), pages 1-8, May.
    • Handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms4664
    DOI: 10.1038/ncomms4664
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    Cited by:

    1. Tal M. Dankovich & Rahul Kaushik & Linda H. M. Olsthoorn & Gabriel Cassinelli Petersen & Philipp Emanuel Giro & Verena Kluever & Paola Agüi-Gonzalez & Katharina Grewe & Guobin Bao & Sabine Beuermann &, 2021. "Extracellular matrix remodeling through endocytosis and resurfacing of Tenascin-R," Nature Communications, Nature, vol. 12(1), pages 1-23, December.
    2. Yunhao Bai & Bokai Zhu & John-Paul Oliveria & Bryan J. Cannon & Dorien Feyaerts & Marc Bosse & Kausalia Vijayaragavan & Noah F. Greenwald & Darci Phillips & Christian M. Schürch & Samuel M. Naik & Edw, 2023. "Expanded vacuum-stable gels for multiplexed high-resolution spatial histopathology," Nature Communications, Nature, vol. 14(1), pages 1-18, December.
    3. Martin Meschkat & Anna M. Steyer & Marie-Theres Weil & Kathrin Kusch & Olaf Jahn & Lars Piepkorn & Paola Agüi-Gonzalez & Nhu Thi Ngoc Phan & Torben Ruhwedel & Boguslawa Sadowski & Silvio O. Rizzoli & , 2022. "White matter integrity in mice requires continuous myelin synthesis at the inner tongue," Nature Communications, Nature, vol. 13(1), pages 1-18, December.

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