IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v5y2014i1d10.1038_ncomms4514.html
   My bibliography  Save this article

Estimating the effective density of engineered nanomaterials for in vitro dosimetry

Author

Listed:
  • Glen DeLoid

    (Center for Nanotechnology and Nanotoxicology, Harvard School of Public Health)

  • Joel M. Cohen

    (Center for Nanotechnology and Nanotoxicology, Harvard School of Public Health)

  • Tom Darrah

    (Nicholas School of the Environment)

  • Raymond Derk

    (National Institute for Occupational Safety and Health, Pathology and Physiology Research Branch)

  • Liying Rojanasakul

    (National Institute for Occupational Safety and Health, Pathology and Physiology Research Branch)

  • Georgios Pyrgiotakis

    (Center for Nanotechnology and Nanotoxicology, Harvard School of Public Health)

  • Wendel Wohlleben

    (BASF SE, GMC/R—G201)

  • Philip Demokritou

    (Center for Nanotechnology and Nanotoxicology, Harvard School of Public Health)

Abstract

The need for accurate in vitro dosimetry remains a major obstacle to the development of cost-effective toxicological screening methods for engineered nanomaterials. An important key to accurate in vitro dosimetry is the characterization of sedimentation and diffusion rates of nanoparticles suspended in culture media, which largely depend upon the effective density and diameter of formed agglomerates in suspension. Here we present a rapid and inexpensive method for accurately measuring the effective density of nano-agglomerates in suspension. This novel method is based on the volume of the pellet obtained by benchtop centrifugation of nanomaterial suspensions in a packed cell volume tube, and is validated against gold-standard analytical ultracentrifugation data. This simple and cost-effective method allows nanotoxicologists to correctly model nanoparticle transport, and thus attain accurate dosimetry in cell culture systems, which will greatly advance the development of reliable and efficient methods for toxicological testing and investigation of nano–bio interactions in vitro.

Suggested Citation

  • Glen DeLoid & Joel M. Cohen & Tom Darrah & Raymond Derk & Liying Rojanasakul & Georgios Pyrgiotakis & Wendel Wohlleben & Philip Demokritou, 2014. "Estimating the effective density of engineered nanomaterials for in vitro dosimetry," Nature Communications, Nature, vol. 5(1), pages 1-10, May.
    • Handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms4514
    DOI: 10.1038/ncomms4514
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/ncomms4514
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/ncomms4514?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Magdiel Inggrid Setyawati & Qin Wang & Nengyi Ni & Jie Kai Tee & Katsuhiko Ariga & Pu Chun Ke & Han Kiat Ho & Yucai Wang & David Tai Leong, 2023. "Engineering tumoral vascular leakiness with gold nanoparticles," Nature Communications, Nature, vol. 14(1), pages 1-15, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms4514. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.