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Imaging of molecular surface dynamics in brain slices using single-particle tracking

Author

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  • B. Biermann

    (Research Group Molecular Physiology, Leibniz-Institute of Neurobiology
    Institute of Anatomy and Molecular Neurobiology, Westfälische Wilhelms-University)

  • S. Sokoll

    (Research Group Molecular Physiology, Leibniz-Institute of Neurobiology)

  • J. Klueva

    (Research Group Molecular Physiology, Leibniz-Institute of Neurobiology)

  • M. Missler

    (Institute of Anatomy and Molecular Neurobiology, Westfälische Wilhelms-University)

  • J. S. Wiegert

    (Center for Molecular Neurobiology (ZMNH), University Medical Center Hamburg-Eppendorf)

  • J. -B. Sibarita

    (University of Bordeaux, Interdisciplinary Institute for Neuroscience
    CNRS UMR 5297)

  • M. Heine

    (Research Group Molecular Physiology, Leibniz-Institute of Neurobiology)

Abstract

Organization of signalling molecules in biological membranes is crucial for cellular communication. Many receptors, ion channels and cell adhesion molecules are associated with proteins important for their trafficking, surface localization or function. These complexes are embedded in a lipid environment of varying composition. Binding affinities and stoichiometry of such complexes were so far experimentally accessible only in isolated systems or monolayers of cell culture. Visualization of molecular dynamics within signalling complexes and their correlation to specialized membrane compartments demand high temporal and spatial resolution and has been difficult to demonstrate in complex tissue like brain slices. Here we demonstrate the feasibility of single-particle tracking (SPT) in organotypic brain slices to measure molecular dynamics of lipids and transmembrane proteins in correlation to synaptic membrane compartments. This method will provide important information about the dynamics and organization of surface molecules in the complex environment of neuronal networks within brain slices.

Suggested Citation

  • B. Biermann & S. Sokoll & J. Klueva & M. Missler & J. S. Wiegert & J. -B. Sibarita & M. Heine, 2014. "Imaging of molecular surface dynamics in brain slices using single-particle tracking," Nature Communications, Nature, vol. 5(1), pages 1-10, May.
    • Handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms4024
    DOI: 10.1038/ncomms4024
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    Cited by:

    1. Andrea Bucci & Giorgio Tortarolo & Marcus Oliver Held & Luca Bega & Eleonora Perego & Francesco Castagnetti & Irene Bozzoni & Eli Slenders & Giuseppe Vicidomini, 2024. "4D Single-particle tracking with asynchronous read-out single-photon avalanche diode array detector," Nature Communications, Nature, vol. 15(1), pages 1-12, December.
    2. Rahul Gupta, 2018. "Self-crowding of AMPA receptors in the excitatory postsynaptic density can effectuate anomalous receptor sub-diffusion," PLOS Computational Biology, Public Library of Science, vol. 14(2), pages 1-34, February.

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