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MEIOB exhibits single-stranded DNA-binding and exonuclease activities and is essential for meiotic recombination

Author

Listed:
  • Mengcheng Luo

    (Center for Animal Transgenesis and Germ Cell Research, University of Pennsylvania School of Veterinary Medicine)

  • Fang Yang

    (Center for Animal Transgenesis and Germ Cell Research, University of Pennsylvania School of Veterinary Medicine)

  • N. Adrian Leu

    (Center for Animal Transgenesis and Germ Cell Research, University of Pennsylvania School of Veterinary Medicine)

  • Jessica Landaiche

    (Chicago College of Osteopathic Medicine, Midwestern University)

  • Mary Ann Handel

    (The Jackson Laboratory)

  • Ricardo Benavente

    (Biocenter of the University of Würzburg)

  • Sophie La Salle

    (Chicago College of Osteopathic Medicine, Midwestern University)

  • P. Jeremy Wang

    (Center for Animal Transgenesis and Germ Cell Research, University of Pennsylvania School of Veterinary Medicine)

Abstract

Meiotic recombination enables the reciprocal exchange of genetic material between parental homologous chromosomes, and ensures faithful chromosome segregation during meiosis in sexually reproducing organisms. This process relies on the complex interaction of DNA repair factors and many steps remain poorly understood in mammals. Here we report the identification of MEIOB, a meiosis-specific protein, in a proteomics screen for novel meiotic chromatin-associated proteins in mice. MEIOB contains an OB domain with homology to one of the RPA1 OB folds. MEIOB binds to single-stranded DNA and exhibits 3′–5′ exonuclease activity. MEIOB forms a complex with RPA and with SPATA22, and these three proteins co-localize in foci that are associated with meiotic chromosomes. Strikingly, chromatin localization and stability of MEIOB depends on SPATA22 and vice versa. Meiob-null mouse mutants exhibit a failure in meiosis and sterility in both sexes. Our results suggest that MEIOB is required for meiotic recombination and chromosomal synapsis.

Suggested Citation

  • Mengcheng Luo & Fang Yang & N. Adrian Leu & Jessica Landaiche & Mary Ann Handel & Ricardo Benavente & Sophie La Salle & P. Jeremy Wang, 2013. "MEIOB exhibits single-stranded DNA-binding and exonuclease activities and is essential for meiotic recombination," Nature Communications, Nature, vol. 4(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:4:y:2013:i:1:d:10.1038_ncomms3788
    DOI: 10.1038/ncomms3788
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    Cited by:

    1. Masaru Ito & Asako Furukohri & Kenichiro Matsuzaki & Yurika Fujita & Atsushi Toyoda & Akira Shinohara, 2023. "FIGNL1 AAA+ ATPase remodels RAD51 and DMC1 filaments in pre-meiotic DNA replication and meiotic recombination," Nature Communications, Nature, vol. 14(1), pages 1-19, December.
    2. Mengwen Hu & Yu-Han Yeh & Yasuhisa Munakata & Hironori Abe & Akihiko Sakashita & So Maezawa & Miguel Vidal & Haruhiko Koseki & Neil Hunter & Richard M. Schultz & Satoshi H. Namekawa, 2022. "PRC1-mediated epigenetic programming is required to generate the ovarian reserve," Nature Communications, Nature, vol. 13(1), pages 1-10, December.
    3. Manickam Gurusaran & Jingjing Zhang & Kexin Zhang & Hiroki Shibuya & Owen R. Davies, 2024. "MEILB2-BRME1 forms a V-shaped DNA clamp upon BRCA2-binding in meiotic recombination," Nature Communications, Nature, vol. 15(1), pages 1-16, December.

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