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Molecular mechanism of strigolactone perception by DWARF14

Author

Listed:
  • Hidemitsu Nakamura

    (Graduate School of Agricultural and Life Sciences, The University of Tokyo)

  • You-Lin Xue

    (Graduate School of Agricultural and Life Sciences, The University of Tokyo)

  • Takuya Miyakawa

    (Graduate School of Agricultural and Life Sciences, The University of Tokyo)

  • Feng Hou

    (Graduate School of Agricultural and Life Sciences, The University of Tokyo)

  • Hui-Min Qin

    (Graduate School of Agricultural and Life Sciences, The University of Tokyo)

  • Kosuke Fukui

    (Graduate School of Agricultural and Life Sciences, The University of Tokyo)

  • Xuan Shi

    (Graduate School of Agricultural and Life Sciences, The University of Tokyo)

  • Emi Ito

    (Graduate School of Science, The University of Tokyo)

  • Shinsaku Ito

    (Graduate School of Agricultural and Life Sciences, The University of Tokyo)

  • Seung-Hyun Park

    (Graduate School of Agricultural and Life Sciences, The University of Tokyo)

  • Yumiko Miyauchi

    (Graduate School of Agricultural and Life Sciences, The University of Tokyo)

  • Atsuko Asano

    (Graduate School of Agricultural and Life Sciences, The University of Tokyo)

  • Naoya Totsuka

    (Graduate School of Agricultural and Life Sciences, The University of Tokyo)

  • Takashi Ueda

    (Graduate School of Science, The University of Tokyo)

  • Masaru Tanokura

    (Graduate School of Agricultural and Life Sciences, The University of Tokyo)

  • Tadao Asami

    (Graduate School of Agricultural and Life Sciences, The University of Tokyo
    JST, CREST, 4-1-8 Honcho, Kawaguchi)

Abstract

Strigolactones (SLs) are phytohormones that inhibit shoot branching and function in the rhizospheric communication with symbiotic fungi and parasitic weeds. An α/β-hydrolase protein, DWARF14 (D14), has been recognized to be an essential component of plant SL signalling, although its precise function remains unknown. Here we present the SL-dependent interaction of D14 with a gibberellin signalling repressor SLR1 and a possible mechanism of phytohormone perception in D14-mediated SL signalling. D14 functions as a cleavage enzyme of SLs, and the cleavage reaction induces the interaction with SLR1. The crystal structure of D14 shows that 5-hydroxy-3-methylbutenolide (D-OH), which is a reaction product of SLs, is trapped in the catalytic cavity of D14 to form an altered surface. The D14 residues recognizing D-OH are critical for the SL-dependent D14−SLR1 interaction. These results provide new insight into crosstalk between gibberellin and SL signalling pathways.

Suggested Citation

  • Hidemitsu Nakamura & You-Lin Xue & Takuya Miyakawa & Feng Hou & Hui-Min Qin & Kosuke Fukui & Xuan Shi & Emi Ito & Shinsaku Ito & Seung-Hyun Park & Yumiko Miyauchi & Atsuko Asano & Naoya Totsuka & Taka, 2013. "Molecular mechanism of strigolactone perception by DWARF14," Nature Communications, Nature, vol. 4(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:4:y:2013:i:1:d:10.1038_ncomms3613
    DOI: 10.1038/ncomms3613
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    Cited by:

    1. Dawei Wang & Zhili Pang & Haiyang Yu & Benjamin Thiombiano & Aimee Walmsley & Shuyi Yu & Yingying Zhang & Tao Wei & Lu Liang & Jing Wang & Xin Wen & Harro J. Bouwmeester & Ruifeng Yao & Zhen Xi, 2022. "Probing strigolactone perception mechanisms with rationally designed small-molecule agonists stimulating germination of root parasitic weeds," Nature Communications, Nature, vol. 13(1), pages 1-12, December.

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