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Torque modulates nucleosome stability and facilitates H2A/H2B dimer loss

Author

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  • Maxim Y. Sheinin

    (Cornell University)

  • Ming Li

    (Cornell University)

  • Mohammad Soltani

    (Cornell University
    Howard Hughes Medical Institute, Cornell University)

  • Karolin Luger

    (Colorado State University)

  • Michelle D. Wang

    (Cornell University
    Howard Hughes Medical Institute, Cornell University)

Abstract

The nucleosome, the fundamental packing unit of chromatin, has a distinct chirality: 147 bp of DNA are wrapped around the core histones in a left-handed, negative superhelix. It has been suggested that this chirality has functional significance, particularly in the context of the cellular processes that generate DNA supercoiling, such as transcription and replication. However, the impact of torsion on nucleosome structure and stability is largely unknown. Here we perform a detailed investigation of single nucleosome behaviour on the high-affinity 601-positioning sequence under tension and torque using the angular optical trapping technique. We find that torque has only a moderate effect on nucleosome unwrapping. In contrast, we observe a dramatic loss of H2A/H2B dimers on nucleosome disruption under positive torque, whereas (H3/H4)2 tetramers are efficiently retained irrespective of torsion. These data indicate that torque could regulate histone exchange during transcription and replication.

Suggested Citation

  • Maxim Y. Sheinin & Ming Li & Mohammad Soltani & Karolin Luger & Michelle D. Wang, 2013. "Torque modulates nucleosome stability and facilitates H2A/H2B dimer loss," Nature Communications, Nature, vol. 4(1), pages 1-8, December.
  • Handle: RePEc:nat:natcom:v:4:y:2013:i:1:d:10.1038_ncomms3579
    DOI: 10.1038/ncomms3579
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    Cited by:

    1. Humberto Sánchez & Zhaowei Liu & Edo Veen & Theo Laar & John F. X. Diffley & Nynke H. Dekker, 2023. "A chromatinized origin reduces the mobility of ORC and MCM through interactions and spatial constraint," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    2. Jaeyoon Lee & Meiling Wu & James T. Inman & Gundeep Singh & Seong ha Park & Joyce H. Lee & Robert M. Fulbright & Yifeng Hong & Joshua Jeong & James M. Berger & Michelle D. Wang, 2023. "Chromatinization modulates topoisomerase II processivity," Nature Communications, Nature, vol. 14(1), pages 1-17, December.
    3. Joana Segura & Ofelia Díaz-Ingelmo & Belén Martínez-García & Alba Ayats-Fraile & Christoforos Nikolaou & Joaquim Roca, 2024. "Nucleosomal DNA has topological memory," Nature Communications, Nature, vol. 15(1), pages 1-10, December.

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