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Darwinian evolution in a translation-coupled RNA replication system within a cell-like compartment

Author

Listed:
  • Norikazu Ichihashi

    (Exploratory Research for Advanced Technology, Japan Science and Technology Agency, Osaka University
    Graduate School of Information Science and Technology, Osaka University)

  • Kimihito Usui

    (Exploratory Research for Advanced Technology, Japan Science and Technology Agency, Osaka University)

  • Yasuaki Kazuta

    (Exploratory Research for Advanced Technology, Japan Science and Technology Agency, Osaka University)

  • Takeshi Sunami

    (Exploratory Research for Advanced Technology, Japan Science and Technology Agency, Osaka University
    Graduate School of Information Science and Technology, Osaka University)

  • Tomoaki Matsuura

    (Exploratory Research for Advanced Technology, Japan Science and Technology Agency, Osaka University
    Graduate School of Information Science and Technology, Osaka University
    Graduate School of Engineering, Osaka University)

  • Tetsuya Yomo

    (Exploratory Research for Advanced Technology, Japan Science and Technology Agency, Osaka University
    Graduate School of Information Science and Technology, Osaka University
    Graduate School of Frontier Biosciences, Osaka University)

Abstract

The ability to evolve is a key characteristic that distinguishes living things from non-living chemical compounds. The construction of an evolvable cell-like system entirely from non-living molecules has been a major challenge. Here we construct an evolvable artificial cell model from an assembly of biochemical molecules. The artificial cell model contains artificial genomic RNA that replicates through the translation of its encoded RNA replicase. We perform a long-term (600-generation) replication experiment using this system, in which mutations are spontaneously introduced into the RNA by replication error, and highly replicable mutants dominate the population according to Darwinian principles. During evolution, the genomic RNA gradually reinforces its interaction with the translated replicase, thereby acquiring competitiveness against selfish (parasitic) RNAs. This study provides the first experimental evidence that replicating systems can be developed through Darwinian evolution in a cell-like compartment, even in the presence of parasitic replicators.

Suggested Citation

  • Norikazu Ichihashi & Kimihito Usui & Yasuaki Kazuta & Takeshi Sunami & Tomoaki Matsuura & Tetsuya Yomo, 2013. "Darwinian evolution in a translation-coupled RNA replication system within a cell-like compartment," Nature Communications, Nature, vol. 4(1), pages 1-7, December.
    • Handle: RePEc:nat:natcom:v:4:y:2013:i:1:d:10.1038_ncomms3494
    DOI: 10.1038/ncomms3494
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    Cited by:

    1. Tony Z. Jia & Yutetsu Kuruma, 2019. "Recent Advances in Origins of Life Research by Biophysicists in Japan," Challenges, MDPI, vol. 10(1), pages 1-21, April.
    2. Zhanar Abil & Ana María Restrepo Sierra & Andreea R. Stan & Amélie Châne & Alicia Prado & Miguel Vega & Yannick Rondelez & Christophe Danelon, 2024. "Darwinian Evolution of Self-Replicating DNA in a Synthetic Protocell," Nature Communications, Nature, vol. 15(1), pages 1-15, December.

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