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A versatile cis-acting inverter module for synthetic translational switches

Author

Listed:
  • Kei Endo

    (International Cooperative Research Project, Japan Science and Technology Agency
    Center for iPS Cell Research and Application, Kyoto University)

  • Karin Hayashi

    (International Cooperative Research Project, Japan Science and Technology Agency
    Center for iPS Cell Research and Application, Kyoto University)

  • Tan Inoue

    (International Cooperative Research Project, Japan Science and Technology Agency
    Laboratory of Gene Biodynamics, Graduate School of Biostudies, Kyoto University)

  • Hirohide Saito

    (International Cooperative Research Project, Japan Science and Technology Agency
    Center for iPS Cell Research and Application, Kyoto University
    The Hakubi Center for Advanced Research, Kyoto University)

Abstract

Artificial genetic switches have been designed and tuned individually in living cells. A method to directly invert an existing OFF switch to an ON switch should be highly convenient to construct complex circuits from well-characterized modules, but developing such a technique has remained a challenge. Here we present a cis-acting RNA module to invert the function of a synthetic translational OFF switch to an ON switch in mammalian cells. This inversion maintains the property of the parental switch in response to a particular input signal. In addition, we demonstrate simultaneous and specific expression control of both the OFF and ON switches. The module fits the criteria of universality and expands the versatility of mRNA-based information processing systems developed for artificially controlling mammalian cellular behaviour.

Suggested Citation

  • Kei Endo & Karin Hayashi & Tan Inoue & Hirohide Saito, 2013. "A versatile cis-acting inverter module for synthetic translational switches," Nature Communications, Nature, vol. 4(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:4:y:2013:i:1:d:10.1038_ncomms3393
    DOI: 10.1038/ncomms3393
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    Cited by:

    1. Shunsuke Kawasaki & Hiroki Ono & Moe Hirosawa & Takeru Kuwabara & Shunsuke Sumi & Suji Lee & Knut Woltjen & Hirohide Saito, 2023. "Programmable mammalian translational modulators by CRISPR-associated proteins," Nature Communications, Nature, vol. 14(1), pages 1-17, December.

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