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The RAG2 C-terminus and ATM protect genome integrity by controlling antigen receptor gene cleavage

Author

Listed:
  • Julie Chaumeil

    (New York University School of Medicine)

  • Mariann Micsinai

    (New York University School of Medicine
    Center for Health Informatics and Bioinformatics, New York University School of Medicine
    NYU Cancer Institute, New York University School of Medicine
    Yale University School of Medicine)

  • Panagiotis Ntziachristos

    (New York University School of Medicine)

  • David B. Roth

    (Abramson Family Cancer Research Institute, Raymond and Ruth Perelman School of Medicine, University of Pennsylvania)

  • Iannis Aifantis

    (New York University School of Medicine
    Howard Hughes Medical Institute, New York University School of Medicine)

  • Yuval Kluger

    (Yale University School of Medicine)

  • Ludovic Deriano

    (Institut Pasteur, CNRS-URA1961
    Institut Pasteur, CNRS-URA1961)

  • Jane A. Skok

    (New York University School of Medicine
    NYU Cancer Institute, New York University School of Medicine)

Abstract

Tight control of antigen-receptor gene rearrangement is required to preserve genome integrity and prevent the occurrence of leukaemia and lymphoma. Nonetheless, mistakes can happen, leading to the generation of aberrant rearrangements, such as Tcra/d–Igh inter-locus translocations that are a hallmark of ataxia telangiectasia-mutated (ATM) deficiency. Current evidence indicates that these translocations arise from the persistence of unrepaired breaks converging at different stages of thymocyte differentiation. Here we show that a defect in feedback control of RAG2 activity gives rise to bi-locus breaks and damage on Tcra/d and Igh in the same T cell at the same developmental stage, which provides a direct mechanism for generating these inter-locus rearrangements. Both the RAG2 C-terminus and ATM prevent bi-locus RAG-mediated cleavage through modulation of three-dimensional conformation (higher-order loops) and nuclear organization of the two loci. This limits the number of potential substrates for translocation and provides an important mechanism for protecting genome stability.

Suggested Citation

  • Julie Chaumeil & Mariann Micsinai & Panagiotis Ntziachristos & David B. Roth & Iannis Aifantis & Yuval Kluger & Ludovic Deriano & Jane A. Skok, 2013. "The RAG2 C-terminus and ATM protect genome integrity by controlling antigen receptor gene cleavage," Nature Communications, Nature, vol. 4(1), pages 1-10, October.
    • Handle: RePEc:nat:natcom:v:4:y:2013:i:1:d:10.1038_ncomms3231
    DOI: 10.1038/ncomms3231
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    Cited by:

    1. Ramya Raviram & Pedro P Rocha & Christian L Müller & Emily R Miraldi & Sana Badri & Yi Fu & Emily Swanzey & Charlotte Proudhon & Valentina Snetkova & Richard Bonneau & Jane A Skok, 2016. "4C-ker: A Method to Reproducibly Identify Genome-Wide Interactions Captured by 4C-Seq Experiments," PLOS Computational Biology, Public Library of Science, vol. 12(3), pages 1-23, March.

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