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High-speed panoramic light-sheet microscopy reveals global endodermal cell dynamics

Author

Listed:
  • Benjamin Schmid

    (Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstr. 108)

  • Gopi Shah

    (Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstr. 108)

  • Nico Scherf

    (Institute for Medical Informatics and Biometry, Medical School, TU Dresden, Fetscherstr. 74)

  • Michael Weber

    (Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstr. 108)

  • Konstantin Thierbach

    (Institute for Medical Informatics and Biometry, Medical School, TU Dresden, Fetscherstr. 74)

  • Citlali Pérez Campos

    (Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstr. 108)

  • Ingo Roeder

    (Institute for Medical Informatics and Biometry, Medical School, TU Dresden, Fetscherstr. 74)

  • Pia Aanstad

    (Institute of Molecular Biology and Center for Molecular Biosciences Innsbruck, University of Innsbruck, Technikerstr. 25)

  • Jan Huisken

    (Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstr. 108)

Abstract

The ever-increasing speed and resolution of modern microscopes make the storage and post-processing of images challenging and prevent thorough statistical analyses in developmental biology. Here, instead of deploying massive storage and computing power, we exploit the spherical geometry of zebrafish embryos by computing a radial maximum intensity projection in real time with a 240-fold reduction in data rate. In our four-lens selective plane illumination microscope (SPIM) setup the development of multiple embryos is recorded in parallel and a map of all labelled cells is obtained for each embryo in

Suggested Citation

  • Benjamin Schmid & Gopi Shah & Nico Scherf & Michael Weber & Konstantin Thierbach & Citlali Pérez Campos & Ingo Roeder & Pia Aanstad & Jan Huisken, 2013. "High-speed panoramic light-sheet microscopy reveals global endodermal cell dynamics," Nature Communications, Nature, vol. 4(1), pages 1-10, October.
    • Handle: RePEc:nat:natcom:v:4:y:2013:i:1:d:10.1038_ncomms3207
    DOI: 10.1038/ncomms3207
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    Cited by:

    1. Karin D. Prummel & Helena L. Crowell & Susan Nieuwenhuize & Eline C. Brombacher & Stephan Daetwyler & Charlotte Soneson & Jelena Kresoja-Rakic & Agnese Kocere & Manuel Ronner & Alexander Ernst & Zahra, 2022. "Hand2 delineates mesothelium progenitors and is reactivated in mesothelioma," Nature Communications, Nature, vol. 13(1), pages 1-21, December.
    2. John Fadul & Teresa Zulueta-Coarasa & Gloria M. Slattum & Nadja M. Redd & Mauricio Franco Jin & Michael J. Redd & Stephan Daetwyler & Danielle Hedeen & Jan Huisken & Jody Rosenblatt, 2021. "KRas-transformed epithelia cells invade and partially dedifferentiate by basal cell extrusion," Nature Communications, Nature, vol. 12(1), pages 1-9, December.
    3. Bingying Chen & Bo-Jui Chang & Stephan Daetwyler & Felix Zhou & Shiv Sharma & Donghoon M. Lee & Amruta Nayak & Jungsik Noh & Konstantin Dubrovinski & Elizabeth H. Chen & Michael Glotzer & Reto Fiolka, 2024. "Projective light-sheet microscopy with flexible parameter selection," Nature Communications, Nature, vol. 15(1), pages 1-8, December.
    4. Xiaomeng Han & Xiaotang Lu & Peter H. Li & Shuohong Wang & Richard Schalek & Yaron Meirovitch & Zudi Lin & Jason Adhinarta & Karl D. Murray & Leah M. MacNiven & Daniel R. Berger & Yuelong Wu & Tao Fan, 2024. "Multiplexed volumetric CLEM enabled by scFvs provides insights into the cytology of cerebellar cortex," Nature Communications, Nature, vol. 15(1), pages 1-18, December.

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