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A DNA tweezer-actuated enzyme nanoreactor

Author

Listed:
  • Minghui Liu

    (Center for Single Molecule Biophysics, Biodesign Institute, Arizona State University
    Arizona State University)

  • Jinglin Fu

    (Center for Single Molecule Biophysics, Biodesign Institute, Arizona State University
    Center for Innovations in Medicine, Biodesign Institute, Arizona State University)

  • Christian Hejesen

    (Centre for DNA Nanotechnology, Aarhus University)

  • Yuhe Yang

    (Center for Single Molecule Biophysics, Biodesign Institute, Arizona State University
    Arizona State University)

  • Neal W. Woodbury

    (Arizona State University
    Center for Innovations in Medicine, Biodesign Institute, Arizona State University)

  • Kurt Gothelf

    (Centre for DNA Nanotechnology, Aarhus University)

  • Yan Liu

    (Center for Single Molecule Biophysics, Biodesign Institute, Arizona State University
    Arizona State University)

  • Hao Yan

    (Center for Single Molecule Biophysics, Biodesign Institute, Arizona State University
    Arizona State University)

Abstract

The functions of regulatory enzymes are essential to modulating cellular pathways. Here we report a tweezer-like DNA nanodevice to actuate the activity of an enzyme/cofactor pair. A dehydrogenase and NAD+ cofactor are attached to different arms of the DNA tweezer structure and actuation of enzymatic function is achieved by switching the tweezers between open and closed states. The enzyme/cofactor pair is spatially separated in the open state with inhibited enzyme function, whereas in the closed state, enzyme is activated by the close proximity of the two molecules. The conformational state of the DNA tweezer is controlled by the addition of specific oligonucleotides that serve as the thermodynamic driver (fuel) to trigger the change. Using this approach, several cycles of externally controlled enzyme inhibition and activation are successfully demonstrated. This principle of responsive enzyme nanodevices may be used to regulate other types of enzymes and to introduce feedback or feed-forward control loops.

Suggested Citation

  • Minghui Liu & Jinglin Fu & Christian Hejesen & Yuhe Yang & Neal W. Woodbury & Kurt Gothelf & Yan Liu & Hao Yan, 2013. "A DNA tweezer-actuated enzyme nanoreactor," Nature Communications, Nature, vol. 4(1), pages 1-5, October.
    • Handle: RePEc:nat:natcom:v:4:y:2013:i:1:d:10.1038_ncomms3127
    DOI: 10.1038/ncomms3127
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    Cited by:

    1. Vishal Maingi & Zhao Zhang & Chris Thachuk & Namita Sarraf & Edwin R. Chapman & Paul W. K. Rothemund, 2023. "Digital nanoreactors to control absolute stoichiometry and spatiotemporal behavior of DNA receptors within lipid bilayers," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
    2. Yusuke Takezawa & Keita Mori & Wei-En Huang & Kotaro Nishiyama & Tong Xing & Takahiro Nakama & Mitsuhiko Shionoya, 2023. "Metal-mediated DNA strand displacement and molecular device operations based on base-pair switching of 5-hydroxyuracil nucleobases," Nature Communications, Nature, vol. 14(1), pages 1-10, December.
    3. Swarup Dey & Adam Dorey & Leeza Abraham & Yongzheng Xing & Irene Zhang & Fei Zhang & Stefan Howorka & Hao Yan, 2022. "A reversibly gated protein-transporting membrane channel made of DNA," Nature Communications, Nature, vol. 13(1), pages 1-12, December.

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