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Formin mDia1 senses and generates mechanical forces on actin filaments

Author

Listed:
  • Antoine Jégou

    (Laboratoire d’Enzymologie et Biochimie Structurales, CNRS, avenue de la Terrasse)

  • Marie-France Carlier

    (Laboratoire d’Enzymologie et Biochimie Structurales, CNRS, avenue de la Terrasse)

  • Guillaume Romet-Lemonne

    (Laboratoire d’Enzymologie et Biochimie Structurales, CNRS, avenue de la Terrasse)

Abstract

Cytoskeleton assembly is instrumental in the regulation of biological functions by physical forces. In a number of key cellular processes, actin filaments elongated by formins such as mDia are subject to mechanical tension, yet how mechanical forces modulate the assembly of actin filaments is an open question. Here, using the viscous drag of a microfluidic flow, we apply calibrated piconewton pulling forces to individual actin filaments that are being elongated at their barbed end by surface-anchored mDia1 proteins. We show that mDia1 is mechanosensitive and that the elongation rate of filaments is increased up to two-fold by the application of a pulling force. We also show that mDia1 is able to track a depolymerizing barbed end in spite of an opposing pulling force, which means that mDia1 can efficiently put actin filaments under mechanical tension. Our findings suggest that formin function in cells is tightly coupled to the mechanical activity of other machineries.

Suggested Citation

  • Antoine Jégou & Marie-France Carlier & Guillaume Romet-Lemonne, 2013. "Formin mDia1 senses and generates mechanical forces on actin filaments," Nature Communications, Nature, vol. 4(1), pages 1-7, October.
    • Handle: RePEc:nat:natcom:v:4:y:2013:i:1:d:10.1038_ncomms2888
    DOI: 10.1038/ncomms2888
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    Cited by:

    1. Sayaka Sekine & Mitsusuke Tarama & Housei Wada & Mustafa M. Sami & Tatsuo Shibata & Shigeo Hayashi, 2024. "Emergence of periodic circumferential actin cables from the anisotropic fusion of actin nanoclusters during tubulogenesis," Nature Communications, Nature, vol. 15(1), pages 1-13, December.

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