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Spatial segregation of polarity factors into distinct cortical clusters is required for cell polarity control

Author

Listed:
  • James Dodgson

    (The Gurdon Institute, University of Cambridge)

  • Anatole Chessel

    (The Gurdon Institute, University of Cambridge)

  • Miki Yamamoto

    (University of Tokyo)

  • Federico Vaggi

    (The Microsoft Research-University of Trento Centre for Computational Systems Biology, Piazza Manifattura 1, Rovereto 38068, Italy)

  • Susan Cox

    (New Hunt's House, King’s College London)

  • Edward Rosten

    (University of Cambridge)

  • David Albrecht

    (Institute of Biochemistry, ETH Zurich, Schafmattstrasse 18, HPM G16.2, Zurich CH-8093, Switzerland)

  • Marco Geymonat

    (The Gurdon Institute, University of Cambridge)

  • Attila Csikasz-Nagy

    (The Microsoft Research-University of Trento Centre for Computational Systems Biology, Piazza Manifattura 1, Rovereto 38068, Italy
    New Hunt's House, King’s College London
    Present address: Research and Innovation Center, Fondazione Edmund Mach, San Michele all’Adige 38010, Italy)

  • Masamitsu Sato

    (University of Tokyo)

  • Rafael E. Carazo-Salas

    (The Gurdon Institute, University of Cambridge
    Institute of Biochemistry, ETH Zurich, Schafmattstrasse 18, HPM G16.2, Zurich CH-8093, Switzerland)

Abstract

Cell polarity is regulated by evolutionarily conserved polarity factors whose precise higher-order organization at the cell cortex is largely unknown. Here we image frontally the cortex of live fission yeast cells using time-lapse and super-resolution microscopy. Interestingly, we find that polarity factors are organized in discrete cortical clusters resolvable to ~50–100 nm in size, which can form and become cortically enriched by oligomerization. We show that forced co-localization of the polarity factors Tea1 and Tea3 results in polarity defects, suggesting that the maintenance of both factors in distinct clusters is required for polarity. However, during mitosis, their co-localization increases, and Tea3 helps to retain the cortical localization of the Tea1 growth landmark in preparation for growth reactivation following mitosis. Thus, regulated spatial segregation of polarity factor clusters provides a means to spatio-temporally control cell polarity at the cell cortex. We observe similar clusters in Saccharomyces cerevisiae and Caenorhabditis elegans cells, indicating this could be a universal regulatory feature.

Suggested Citation

  • James Dodgson & Anatole Chessel & Miki Yamamoto & Federico Vaggi & Susan Cox & Edward Rosten & David Albrecht & Marco Geymonat & Attila Csikasz-Nagy & Masamitsu Sato & Rafael E. Carazo-Salas, 2013. "Spatial segregation of polarity factors into distinct cortical clusters is required for cell polarity control," Nature Communications, Nature, vol. 4(1), pages 1-9, June.
    • Handle: RePEc:nat:natcom:v:4:y:2013:i:1:d:10.1038_ncomms2813
    DOI: 10.1038/ncomms2813
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    Cited by:

    1. Pekka Ruusuvuori & Lassi Paavolainen & Kalle Rutanen & Anita Mäki & Heikki Huttunen & Varpu Marjomäki, 2014. "Quantitative Analysis of Dynamic Association in Live Biological Fluorescent Samples," PLOS ONE, Public Library of Science, vol. 9(4), pages 1-11, April.
    2. Xi Wang & Fan Zheng & Yuan-yuan Yi & Gao-yuan Wang & Li-xin Hong & Dannel McCollum & Chuanhai Fu & Yamei Wang & Quan-wen Jin, 2022. "Ubiquitination of CLIP-170 family protein restrains polarized growth upon DNA replication stress," Nature Communications, Nature, vol. 13(1), pages 1-14, December.

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