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Regulation of asymmetric cell division and polarity by Scribble is not required for humoral immunity

Author

Listed:
  • Edwin D. Hawkins

    (Cancer Immunology Program, Peter MacCallum Cancer Centre
    The University of Melbourne
    Present address: Imperial College London, Sir Alexander Fleming Building, London SW72AZ, UK)

  • Jane Oliaro

    (Cancer Immunology Program, Peter MacCallum Cancer Centre
    The University of Melbourne)

  • Axel Kallies

    (The Walter and Eliza Hall Institute of Medical Research)

  • Gabrielle T. Belz

    (The Walter and Eliza Hall Institute of Medical Research)

  • Andrew Filby

    (FACS Laboratory, London Research Institute, Cancer Research UK, 44 Lincolns Inn Fields)

  • Thea Hogan

    (MRC National Institute for Medical Research, The Ridgeway, Mill Hill)

  • Nicole Haynes

    (Cancer Immunology Program, Peter MacCallum Cancer Centre
    The University of Melbourne)

  • Kelly M. Ramsbottom

    (Cancer Immunology Program, Peter MacCallum Cancer Centre
    The University of Melbourne)

  • Vanessa Van Ham

    (Cancer Immunology Program, Peter MacCallum Cancer Centre
    The University of Melbourne)

  • Tanja Kinwell

    (The University of Melbourne)

  • Benedict Seddon

    (MRC National Institute for Medical Research, The Ridgeway, Mill Hill)

  • Derek Davies

    (FACS Laboratory, London Research Institute, Cancer Research UK, 44 Lincolns Inn Fields)

  • David Tarlinton

    (The Walter and Eliza Hall Institute of Medical Research)

  • Andrew M. Lew

    (The Walter and Eliza Hall Institute of Medical Research)

  • Patrick O. Humbert

    (The University of Melbourne)

  • Sarah M. Russell

    (Cancer Immunology Program, Peter MacCallum Cancer Centre
    The University of Melbourne
    Centre for Micro-Photonics, Swinburne University of Technology)

Abstract

The production of protective antibody requires effective signalling of naive B cells following encounter with antigen, and the divergence of responding B lymphocytes into distinct lineages. Polarity proteins have recently been proposed as important mediators of both the initial B cell response, and potentially of asymmetric cell division. Here we show that, although polarity proteins of the Scribble complex, Scribble, Dlg1 and Lgl1, are expressed and polarized during early B cell activation, their deficiency has no effect on the in vivo outcome of immunization or challenge with influenza infection. Furthermore, we find a striking correlation in the differentiation outcome of daughters of single founder B cells in vitro. Taken together, our results indicate that B cell differentiation does not require polarity proteins of the Scribble complex, and the findings do not support a role for asymmetric cell division in B cell activation and differentiation.

Suggested Citation

  • Edwin D. Hawkins & Jane Oliaro & Axel Kallies & Gabrielle T. Belz & Andrew Filby & Thea Hogan & Nicole Haynes & Kelly M. Ramsbottom & Vanessa Van Ham & Tanja Kinwell & Benedict Seddon & Derek Davies &, 2013. "Regulation of asymmetric cell division and polarity by Scribble is not required for humoral immunity," Nature Communications, Nature, vol. 4(1), pages 1-11, June.
    • Handle: RePEc:nat:natcom:v:4:y:2013:i:1:d:10.1038_ncomms2796
    DOI: 10.1038/ncomms2796
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    Cited by:

    1. Raz Shimoni & Kim Pham & Mohammed Yassin & Mandy J Ludford-Menting & Min Gu & Sarah M Russell, 2014. "Normalized Polarization Ratios for the Analysis of Cell Polarity," PLOS ONE, Public Library of Science, vol. 9(6), pages 1-11, June.

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