IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v4y2013i1d10.1038_ncomms2725.html
   My bibliography  Save this article

Calcium-dependent permeabilization of erythrocytes by a perforin-like protein during egress of malaria parasites

Author

Listed:
  • Swati Garg

    (Malaria Group, International Centre for Genetic Engineering and Biotechnology (ICGEB))

  • Shalini Agarwal

    (Malaria Group, International Centre for Genetic Engineering and Biotechnology (ICGEB))

  • Saravanan Kumar

    (Plant Biology: Plant Transformation Group, International Centre for Genetic Engineering and Biotechnology (ICGEB))

  • Syed Shams Yazdani

    (Synthetic Biology and Biofuels Group, International Centre for Genetic Engineering and Biotechnology (ICGEB))

  • Chetan E. Chitnis

    (Malaria Group, International Centre for Genetic Engineering and Biotechnology (ICGEB))

  • Shailja Singh

    (Malaria Group, International Centre for Genetic Engineering and Biotechnology (ICGEB)
    Shiv Nadar University)

Abstract

Clinical malaria is associated with proliferation of blood-stage parasites. During the blood stage, Plasmodium parasites invade host red blood cells, multiply, egress and reinvade uninfected red blood cells to continue the life cycle. Here we demonstrate that calcium-dependent permeabilization of host red blood cells is critical for egress of Plasmodium falciparum merozoites. Although perforin-like proteins have been predicted to mediate membrane perforation during egress, the expression, activity and mechanism of action of these proteins have not been demonstrated. Here, we show that two perforin-like proteins, perforin-like protein 1 and perforin-like protein 2, are expressed in the blood stage. Perforin-like protein 1 localizes to the red blood cell membrane and parasitophorous vacuolar membrane in mature schizonts following its Ca2+-dependent discharge from micronemes. Furthermore, perforin-like protein 1 shows Ca2+-dependent permeabilization and membranolytic activities suggesting that it may be one of the effector proteins that mediate Ca2+-dependent membrane perforation during egress.

Suggested Citation

  • Swati Garg & Shalini Agarwal & Saravanan Kumar & Syed Shams Yazdani & Chetan E. Chitnis & Shailja Singh, 2013. "Calcium-dependent permeabilization of erythrocytes by a perforin-like protein during egress of malaria parasites," Nature Communications, Nature, vol. 4(1), pages 1-12, June.
    • Handle: RePEc:nat:natcom:v:4:y:2013:i:1:d:10.1038_ncomms2725
    DOI: 10.1038/ncomms2725
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/ncomms2725
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/ncomms2725?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Fang Jiao & François Dehez & Tao Ni & Xiulian Yu & Jeremy S. Dittman & Robert Gilbert & Christophe Chipot & Simon Scheuring, 2022. "Perforin-2 clockwise hand-over-hand pre-pore to pore transition mechanism," Nature Communications, Nature, vol. 13(1), pages 1-13, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:4:y:2013:i:1:d:10.1038_ncomms2725. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.