IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v4y2013i1d10.1038_ncomms2635.html
   My bibliography  Save this article

Quantum dot imaging platform for single-cell molecular profiling

Author

Listed:
  • Pavel Zrazhevskiy

    (University of Washington, 3720 15th Avenue NE, Seattle, Washington 98195, USA)

  • Xiaohu Gao

    (University of Washington, 3720 15th Avenue NE, Seattle, Washington 98195, USA)

Abstract

Study of normal cell physiology and disease pathogenesis heavily relies on untangling the complexity of intracellular molecular mechanisms and pathways. To achieve this goal, comprehensive molecular profiling of individual cells within the context of microenvironment is required. Here we report the development of a multicolour multicycle in situ imaging technology capable of creating detailed quantitative molecular profiles for individual cells at the resolution of optical imaging. A library of stoichiometric fluorescent probes is prepared by linking target-specific antibodies to a universal quantum dot-based platform via protein A in a quick and simple procedure. Surprisingly, despite the potential for multivalent binding between protein A and antibody and the intermediate affinity of this non-covalent bond, fully assembled probes do not aggregate or exchange antibodies, facilitating highly multiplexed parallel staining. This single-cell molecular profiling technology is expected to open new opportunities in systems biology, gene expression studies, signalling pathway analysis and molecular diagnostics.

Suggested Citation

  • Pavel Zrazhevskiy & Xiaohu Gao, 2013. "Quantum dot imaging platform for single-cell molecular profiling," Nature Communications, Nature, vol. 4(1), pages 1-12, June.
    • Handle: RePEc:nat:natcom:v:4:y:2013:i:1:d:10.1038_ncomms2635
    DOI: 10.1038/ncomms2635
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/ncomms2635
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/ncomms2635?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Jinyoung Kang & Margaret E. Schroeder & Youngmi Lee & Chaitanya Kapoor & Eunah Yu & Tyler B. Tarr & Kat Titterton & Menglong Zeng & Demian Park & Emily Niederst & Donglai Wei & Guoping Feng & Edward S, 2024. "Multiplexed expansion revealing for imaging multiprotein nanostructures in healthy and diseased brain," Nature Communications, Nature, vol. 15(1), pages 1-17, December.
    2. Liwei Yang & Avery Ball & Jesse Liu & Tanya Jain & Yue-Ming Li & Firoz Akhter & Donghui Zhu & Jun Wang, 2022. "Cyclic microchip assay for measurement of hundreds of functional proteins in single neurons," Nature Communications, Nature, vol. 13(1), pages 1-11, December.
    3. Daniel P Riordan & Sushama Varma & Robert B West & Patrick O Brown, 2015. "Automated Analysis and Classification of Histological Tissue Features by Multi-Dimensional Microscopic Molecular Profiling," PLOS ONE, Public Library of Science, vol. 10(7), pages 1-18, July.
    4. Junyoung Seo & Yeonbo Sim & Jeewon Kim & Hyunwoo Kim & In Cho & Hoyeon Nam & Young-Gyu Yoon & Jae-Byum Chang, 2022. "PICASSO allows ultra-multiplexed fluorescence imaging of spatially overlapping proteins without reference spectra measurements," Nature Communications, Nature, vol. 13(1), pages 1-17, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:4:y:2013:i:1:d:10.1038_ncomms2635. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.