IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v4y2013i1d10.1038_ncomms2492.html
   My bibliography  Save this article

Cleavage-based signal amplification of RNA

Author

Listed:
  • Yongyun Zhao

    (Natural Products Research Center, Chengdu Institute of Biology, Chinese Academy of Sciences)

  • Li Zhou

    (Natural Products Research Center, Chengdu Institute of Biology, Chinese Academy of Sciences)

  • Zhuo Tang

    (Natural Products Research Center, Chengdu Institute of Biology, Chinese Academy of Sciences)

Abstract

RNA detection has become an integral part of current biomedical research. Up to now, the reverse transcription–PCR has been the most practical method to detect mRNA targets. However, RNA detection by reverse transcription–PCR requires sophisticated equipment and it is highly sensitive to contamination with genomic DNA. Here we report a new isothermal reaction to simultaneously amplify and detect RNA, based on cleavage by DNAzyme and signal amplification. Cleavage-based signal amplification of RNA cannot be contaminated by genomic DNA and is suitable for the detection of both mRNA and microRNA targets, with high specificity and sensitivity. Moreover, the detection results can be reported in a colorimetric or real-time fluorometric way for different detection purposes.

Suggested Citation

  • Yongyun Zhao & Li Zhou & Zhuo Tang, 2013. "Cleavage-based signal amplification of RNA," Nature Communications, Nature, vol. 4(1), pages 1-8, June.
    • Handle: RePEc:nat:natcom:v:4:y:2013:i:1:d:10.1038_ncomms2492
    DOI: 10.1038/ncomms2492
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/ncomms2492
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/ncomms2492?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Chao Cheng & Qiang Wang & Wenjie You & Manhua Chen & Jiahong Xia, 2014. "MiRNAs as Biomarkers of Myocardial Infarction: A Meta-Analysis," PLOS ONE, Public Library of Science, vol. 9(2), pages 1-11, February.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:4:y:2013:i:1:d:10.1038_ncomms2492. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.