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Self-organization of the vesicular stomatitis virus nucleocapsid into a bullet shape

Author

Listed:
  • Ambroise Desfosses

    (UJF-EMBL-CNRS UMI 3265, Unit of Virus Host Cell Interactions
    Structural and Computational Biology Unit)

  • Euripedes A. Ribeiro

    (UJF-EMBL-CNRS UMI 3265, Unit of Virus Host Cell Interactions)

  • Guy Schoehn

    (UJF-EMBL-CNRS UMI 3265, Unit of Virus Host Cell Interactions
    CEA-CNRS-UJF UMR 5075, Institut de Biologie Structurale-Jean-Pierre Ebel)

  • Danielle Blondel

    (CNRS UPR 3296, Virologie Moléculaire et Structurale)

  • Delphine Guilligay

    (UJF-EMBL-CNRS UMI 3265, Unit of Virus Host Cell Interactions)

  • Marc Jamin

    (UJF-EMBL-CNRS UMI 3265, Unit of Virus Host Cell Interactions)

  • Rob W. H. Ruigrok

    (UJF-EMBL-CNRS UMI 3265, Unit of Virus Host Cell Interactions)

  • Irina Gutsche

    (UJF-EMBL-CNRS UMI 3265, Unit of Virus Host Cell Interactions)

Abstract

The typical bullet shape of Rhabdoviruses is thought to rely on the matrix protein for stabilizing the nucleocapsid coil. Here we scrutinize the morphology of purified and recombinant nucleocapsids of vesicular stomatitis virus in vitro. We elucidate pH and ionic strength conditions for their folding into conical tips and further growth into whole bullets, and provide cryo-electron microscopy reconstructions of the bullet tip and the helical trunk. We address conformational variability of the reconstituted nucleocapsids and the issue of constraints imposed by the binding of matrix protein. Our findings bridge the gap between the isolated nucleoprotein-RNA string in its form of an undulating ribbon, and the tight bullet-shaped virion skeleton.

Suggested Citation

  • Ambroise Desfosses & Euripedes A. Ribeiro & Guy Schoehn & Danielle Blondel & Delphine Guilligay & Marc Jamin & Rob W. H. Ruigrok & Irina Gutsche, 2013. "Self-organization of the vesicular stomatitis virus nucleocapsid into a bullet shape," Nature Communications, Nature, vol. 4(1), pages 1-5, June.
    • Handle: RePEc:nat:natcom:v:4:y:2013:i:1:d:10.1038_ncomms2435
    DOI: 10.1038/ncomms2435
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    Cited by:

    1. Kang Zhou & Zhu Si & Peng Ge & Jun Tsao & Ming Luo & Z. Hong Zhou, 2022. "Atomic model of vesicular stomatitis virus and mechanism of assembly," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    2. Simon Jenni & Joshua A. Horwitz & Louis-Marie Bloyet & Sean P. J. Whelan & Stephen C. Harrison, 2022. "Visualizing molecular interactions that determine assembly of a bullet-shaped vesicular stomatitis virus particle," Nature Communications, Nature, vol. 13(1), pages 1-14, December.

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