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Synthetic RNA devices to expedite the evolution of metabolite-producing microbes

Author

Listed:
  • Jina Yang

    (Pohang University of Science and Technology)

  • Sang Woo Seo

    (Pohang University of Science and Technology)

  • Sungho Jang

    (Pohang University of Science and Technology)

  • So-I Shin

    (Pohang University of Science and Technology)

  • Chae Hyun Lim

    (Pohang University of Science and Technology)

  • Tae-Young Roh

    (Pohang University of Science and Technology
    Pohang University of Science and Technology)

  • Gyoo Yeol Jung

    (Pohang University of Science and Technology
    School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology)

Abstract

An extension of directed evolution strategies to genome-wide variations increases the chance of obtaining metabolite-overproducing microbes. However, a general high-throughput screening platform for selecting improved strains remains out of reach. Here, to expedite the evolution of metabolite-producing microbes, we utilize synthetic RNA devices comprising a riboswitch and a selection module that specifically sense inconspicuous metabolites. Using L-lysine-producing Escherichia coli as a model system, we demonstrated that this RNA device could enrich pathway-optimized strains to up to 75% of the total population after four rounds of enrichment cycles. Furthermore, the potential applicability of this device was examined by successfully extending its application to the case of L-tryptophan. When used in conjunction with combinatorial mutagenesis for metabolite overproduction, our synthetic RNA device should facilitate strain improvement.

Suggested Citation

  • Jina Yang & Sang Woo Seo & Sungho Jang & So-I Shin & Chae Hyun Lim & Tae-Young Roh & Gyoo Yeol Jung, 2013. "Synthetic RNA devices to expedite the evolution of metabolite-producing microbes," Nature Communications, Nature, vol. 4(1), pages 1-7, June.
    • Handle: RePEc:nat:natcom:v:4:y:2013:i:1:d:10.1038_ncomms2404
    DOI: 10.1038/ncomms2404
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    Cited by:

    1. Dae-yeol Ye & Myung Hyun Noh & Jo Hyun Moon & Alfonsina Milito & Minsun Kim & Jeong Wook Lee & Jae-Seong Yang & Gyoo Yeol Jung, 2022. "Kinetic compartmentalization by unnatural reaction for itaconate production," Nature Communications, Nature, vol. 13(1), pages 1-10, December.

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