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Real-time in vivo imaging of the beating mouse heart at microscopic resolution

Author

Listed:
  • Sungon Lee

    (Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Richard B. Simches Research Center
    Interaction and Robotics Research Center, Korea Institute of Science and Technology)

  • Claudio Vinegoni

    (Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Richard B. Simches Research Center)

  • Paolo Fumene Feruglio

    (Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Richard B. Simches Research Center
    Neuropsychological, Morphological and Movement Sciences, University of Verona)

  • Lyuba Fexon

    (Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Richard B. Simches Research Center)

  • Rostic Gorbatov

    (Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Richard B. Simches Research Center)

  • Misha Pivoravov

    (Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Richard B. Simches Research Center)

  • Andrea Sbarbati

    (Neuropsychological, Morphological and Movement Sciences, University of Verona)

  • Matthias Nahrendorf

    (Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Richard B. Simches Research Center)

  • Ralph Weissleder

    (Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Richard B. Simches Research Center)

Abstract

Real-time imaging of moving organs and tissues at microscopic resolutions represents a major challenge in studying the complex biology of live animals. Here we present a technique based on a novel stabilizer setup combined with a gating acquisition algorithm for the imaging of a beating murine heart at the single-cell level. The method allows serial in vivo fluorescence imaging of the beating heart in live mice in both confocal and nonlinear modes over the course of several hours. We demonstrate the utility of this technique for in vivo optical sectioning and dual-channel time-lapse fluorescence imaging of cardiac ischaemia. The generic method could be adapted to other moving organs and thus broadly facilitate in vivo microscopic investigations.

Suggested Citation

  • Sungon Lee & Claudio Vinegoni & Paolo Fumene Feruglio & Lyuba Fexon & Rostic Gorbatov & Misha Pivoravov & Andrea Sbarbati & Matthias Nahrendorf & Ralph Weissleder, 2012. "Real-time in vivo imaging of the beating mouse heart at microscopic resolution," Nature Communications, Nature, vol. 3(1), pages 1-8, January.
    • Handle: RePEc:nat:natcom:v:3:y:2012:i:1:d:10.1038_ncomms2060
    DOI: 10.1038/ncomms2060
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    Cited by:

    1. Randy J Giedt & Peter D Koch & Ralph Weissleder, 2013. "Single Cell Analysis of Drug Distribution by Intravital Imaging," PLOS ONE, Public Library of Science, vol. 8(4), pages 1-9, April.

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