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Rotational dynamics of cargos at pauses during axonal transport

Author

Listed:
  • Yan Gu

    (Ames Laboratory, Iowa State University)

  • Wei Sun

    (Ames Laboratory, Iowa State University
    Present address: Department of Chemistry, the University of Washington, Seattle, Washington 98195, USA.)

  • Gufeng Wang

    (Ames Laboratory, Iowa State University
    Present address: Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695, USA.)

  • Ksenija Jeftinija

    (College of Veterinary Medicine, Iowa State University)

  • Srdija Jeftinija

    (College of Veterinary Medicine, Iowa State University)

  • Ning Fang

    (Ames Laboratory, Iowa State University)

Abstract

Direct visualization of axonal transport in live neurons is essential for our understanding of the neuronal functions and the working mechanisms of microtubule-based motor proteins. Here we use the high-speed single particle orientation and rotational tracking technique to directly visualize the rotational dynamics of cargos in both active directional transport and pausing stages of axonal transport, with a temporal resolution of 2 ms. Both long and short pauses are imaged, and the correlations between the pause duration, the rotational behaviour of the cargo at the pause, and the moving direction after the pause are established. Furthermore, the rotational dynamics leading to switching tracks are visualized in detail. These first-time observations of cargo's rotational dynamics provide new insights on how kinesin and dynein motors take the cargo through the alternating stages of active directional transport and pause.

Suggested Citation

  • Yan Gu & Wei Sun & Gufeng Wang & Ksenija Jeftinija & Srdija Jeftinija & Ning Fang, 2012. "Rotational dynamics of cargos at pauses during axonal transport," Nature Communications, Nature, vol. 3(1), pages 1-8, January.
    • Handle: RePEc:nat:natcom:v:3:y:2012:i:1:d:10.1038_ncomms2037
    DOI: 10.1038/ncomms2037
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    Cited by:

    1. Yanxin Zhang & Rongrong Wen & Jialing Hu & Daoming Guan & Xiaochen Qiu & Yunxiang Zhang & Daniel S. Kohane & Qian Liu, 2022. "Enhancement of single upconversion nanoparticle imaging by topologically segregated core-shell structure with inward energy migration," Nature Communications, Nature, vol. 13(1), pages 1-12, December.

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